Removing of the N-terminal methionine improves the sweetness of the recombinant expressed sweet-tasting protein brazzein and its mutants in Escherichia coli
The sweet-tasting protein brazzein is the smallest candy protein with excessive candy efficiency. Overexpression of this protein in a heterogenous host is a vital means for its manufacturing in meals trade. On this research, the gene of minor kind brazzein was cloned into the pET-SUMO vector with optimized codon utilization and expressed in E. coli BL21-CodonPlus (DE3)-RIL.
The recombinant protein in absence of the N-terminal methionine displayed a sweetness threshold about 1.5 μg/ml, which is the sweetest brazzein protein reported to date. The surprising candy efficiency of the protein was validated by a sequence of mutants (7Val → Arg, 9Glu → Lys and 9Glu → Asp), wherein E9K exhibited about 50% enhancement of sweetness than the wild sort. The superior sweetness of recombinant brazzein and its sweeter mutants counsel their potential purposes in meals and drinks.
PRACTICAL APPLICATIONS: The sweet-tasting proteins are pure, low-, or non-caloric and nutritive, exhibiting to be promising replacers of sugars and synthetic sweeteners, and can be utilized as candy components within the fields of meals, drugs, and biotechnology. Within the current research, we report that the recombinant brazzein protein expressed in E. coli reveals superior and improved sweetness than these beforehand reported.
Moreover, sweeter mutants had been obtained with the expression process. The superior sweetness of recombinant brazzein and its sweeter mutants counsel their potential purposes in meals, drinks, and different fields.
Key phrases: E. coli expression; N-terminus; mutant; sweet-tasting protein brazzein; sweetness
Yeast AHA1 Protein |
|||
| SPR-314C | Stressmarq | 2x0.1 mg | EUR 676.8 |
|
Description: His tagged recombinant yeast AHA1 Protein expressed in E. coli for WB, SDS-PAGE, Functional Assay. The biological activity of this protein has not yet been tested. |
|||
AHA1 protein |
|||
| 30R-1091 | Fitzgerald | 100 ug | EUR 366 |
|
Description: Purified recombinant Human AHA1 protein |
|||
Human AHA1 Protein |
|||
| SPR-300A | Stressmarq | 0.05 mg | EUR 313.2 |
|
Description: Untagged recombinant human AHA1 Protein expressed in E. coli for WB, SDS-PAGE, Functional Assay. The biological activity of this protein has not yet been tested. |
|||
Human AHA1 Protein |
|||
| SPR-300B | Stressmarq | 0.1 mg | EUR 452.4 |
|
Description: Untagged recombinant human AHA1 Protein expressed in E. coli for WB, SDS-PAGE, Functional Assay. The biological activity of this protein has not yet been tested. |
|||
Human AHA1 Protein |
|||
| SPR-300C | Stressmarq | 2x0.1 mg | EUR 676.8 |
|
Description: Untagged recombinant human AHA1 Protein expressed in E. coli for WB, SDS-PAGE, Functional Assay. The biological activity of this protein has not yet been tested. |
|||
Human AHA1 Protein |
|||
| SPR-309A | Stressmarq | 0.05 mg | EUR 313.2 |
|
Description: His tagged recombinant human AHA1 Protein expressed in E. coli for WB, SDS-PAGE, Functional Assay. The biological activity of this protein has not yet been tested. |
|||
Human AHA1 Protein |
|||
| SPR-309B | Stressmarq | 0.1 mg | EUR 452.4 |
|
Description: His tagged recombinant human AHA1 Protein expressed in E. coli for WB, SDS-PAGE, Functional Assay. The biological activity of this protein has not yet been tested. |
|||
Human AHA1 Protein |
|||
| SPR-309C | Stressmarq | 2x0.1 mg | EUR 676.8 |
|
Description: His tagged recombinant human AHA1 Protein expressed in E. coli for WB, SDS-PAGE, Functional Assay. The biological activity of this protein has not yet been tested. |
|||
Mouse AHA1 Protein |
|||
| SPR-311A | Stressmarq | 0.05 mg | EUR 313.2 |
|
Description: His tagged recombinant mouse AHA1 Protein expressed in E. coli for WB, SDS-PAGE, Functional Assay. The biological activity of this protein has not yet been tested. |
|||
Mouse AHA1 Protein |
|||
| SPR-311B | Stressmarq | 0.1 mg | EUR 452.4 |
|
Description: His tagged recombinant mouse AHA1 Protein expressed in E. coli for WB, SDS-PAGE, Functional Assay. The biological activity of this protein has not yet been tested. |
|||
Mouse AHA1 Protein |
|||
| SPR-311C | Stressmarq | 2x0.1 mg | EUR 676.8 |
|
Description: His tagged recombinant mouse AHA1 Protein expressed in E. coli for WB, SDS-PAGE, Functional Assay. The biological activity of this protein has not yet been tested. |
|||
AHA1 antibody |
|||
| 70R-50815 | Fitzgerald | 100 ul | EUR 292.8 |
|
Description: Purified Polyclonal AHA1 antibody |
|||
anti-AHA1 |
|||
| YF-PA17082 | Abfrontier | 50 ul | EUR 435.6 |
|
Description: Mouse polyclonal to AHA1 |
|||
anti-AHA1 |
|||
| YF-PA17083 | Abfrontier | 50 ug | EUR 435.6 |
|
Description: Mouse polyclonal to AHA1 |
|||
anti-AHA1 |
|||
| YF-PA17084 | Abfrontier | 100 ul | EUR 483.6 |
|
Description: Rabbit polyclonal to AHA1 |
|||
anti-AHA1 |
|||
| YF-PA17085 | Abfrontier | 100 ug | EUR 483.6 |
|
Description: Rabbit polyclonal to AHA1 |
|||
Human AHA1 Antibody |
|||
| 32922-05111 | AssayPro | 150 ug | EUR 313.2 |
AHA1 Blocking Peptide |
|||
| 20-abx063690 | Abbexa |
|
|
AHA1 Antibody (Biotin) |
|||
| abx447922-100ul | Abbexa | 100 ul | EUR 661.2 |
AHA1 Antibody (FITC) |
|||
| abx447923-100ul | Abbexa | 100 ul | EUR 661.2 |
AHA1 Antibody (HRP) |
|||
| abx447924-100ul | Abbexa | 100 ul | EUR 644.4 |
Recombinant Yeast IDH1 Protein, Untagged, Yeast-1mg |
|||
| QP10670-1mg | EnQuireBio | 1mg | EUR 186 |
Recombinant Yeast IDH1 Protein, Untagged, Yeast-50mg |
|||
| QP10670-50mg | EnQuireBio | 50mg | EUR 1251.6 |
Recombinant Yeast IDH1 Protein, Untagged, Yeast-5mg |
|||
| QP10670-5mg | EnQuireBio | 5mg | EUR 241.2 |
GLC8 Yeast Recombinant Protein |
|||
| PROTP41818 | BosterBio | Regular: 50ug | EUR 380.4 |
|
Description: GLC8 Yeast (Saccharomyces cerevisiae) Recombinant produced in E.coli is a single, glycosylated polypeptide chain containing 265 amino acids (1-229) and having a molecular mass of 30.7 kDa (molecular size on SDS-PAGE will appear higher). ;The GLC8 is fused to a 35 amino acid His Tag at N-Terminus and purified by standard chromatography techniques. |
|||
AHA1 Activator of HSP90 ATPase-1 Human Recombinant Protein |
|||
| PROTO95433 | BosterBio | Regular: 50ug | EUR 380.4 |
|
Description: AHA1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 320 amino acids (19-337) and having a molecular mass of 36.1 kDa. |
|||
Recombinant Saccharomyces Cerevisiae AHA1 Protein (aa 1-350) [His] |
|||
| VAng-Wyb4128-1mg | Creative Biolabs | 1 mg | EUR 5524.8 |
|
Description: Saccharomyces Cerevisiae (strain ATCC 204508 / S288c) Aha1p protein, recombinant protein. |
|||
Anti-AHA1/AHSA1 Antibody |
|||
| A05733 | BosterBio | 100ug/vial | EUR 352.8 |
Polyclonal AHSA1 / AHA1 Antibody |
|||
| APR14879G | Leading Biology | 0.05ml | EUR 580.8 |
|
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human AHSA1 / AHA1 . This antibody is tested and proven to work in the following applications: |
|||
Anti-AHA1 Monoclonal Antibody |
|||
| M05733-1 | BosterBio | 100ug | EUR 476.4 |
|
Description: Rat Monoclonal AHA1 Antibody. Validated in ELISA, IP, IF, IHC, WB and tested in Human, Mouse, Rat. |
|||
Monoclonal antibody for AHA1 |
|||
| SMC-172C | Stressmarq | 0.025mg | EUR 241.2 |
|
Description: A monoclonal antibody from clone 25F2.D9 against Human | Mouse | Rat AHA1. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Mouse Aha1. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000). This MAb for AHA1 is not conjugated. |
|||
Monoclonal antibody for AHA1 |
|||
| SMC-172D | Stressmarq | 0.1mg | EUR 439.2 |
|
Description: A monoclonal antibody from clone 25F2.D9 against Human | Mouse | Rat AHA1. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Mouse Aha1. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000). This MAb for AHA1 is not conjugated. |
|||
Monoclonal antibody for AHA1 |
|||
| SMC-172D-A390 | Stressmarq | 0.1mg | EUR 495.6 |
|
Description: A monoclonal antibody from clone 25F2.D9 against Human | Mouse | Rat AHA1. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Mouse Aha1. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000). This MAb for AHA1 is conjugated with ATTO 390. |
|||
Monoclonal antibody for AHA1 |
|||
| SMC-172D-A488 | Stressmarq | 0.1mg | EUR 494.4 |
|
Description: A monoclonal antibody from clone 25F2.D9 against Human | Mouse | Rat AHA1. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Mouse Aha1. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000). This MAb for AHA1 is conjugated with ATTO 488. |
|||
Monoclonal antibody for AHA1 |
|||
| SMC-172D-A565 | Stressmarq | 0.1mg | EUR 494.4 |
|
Description: A monoclonal antibody from clone 25F2.D9 against Human | Mouse | Rat | Bovine | Monkey | Hamster | Sheep | Yeast | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) AHA1. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Mouse Aha1. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000). This MAb for AHA1 is conjugated with ATTO 565. |
|||
Monoclonal antibody for AHA1 |
|||
| SMC-172D-A594 | Stressmarq | 0.1mg | EUR 494.4 |
|
Description: A monoclonal antibody from clone 25F2.D9 against Human | Mouse | Rat | Bovine | Monkey | Hamster | Sheep | Yeast | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) AHA1. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Mouse Aha1. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000). This MAb for AHA1 is conjugated with ATTO 594. |
|||
Monoclonal antibody for AHA1 |
|||
| SMC-172D-A633 | Stressmarq | 0.1mg | EUR 494.4 |
|
Description: A monoclonal antibody from clone 25F2.D9 against Human | Mouse | Rat | Bovine | Monkey | Hamster | Sheep | Yeast | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) AHA1. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Mouse Aha1. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000). This MAb for AHA1 is conjugated with ATTO 633. |
|||
Monoclonal antibody for AHA1 |
|||
| SMC-172D-A655 | Stressmarq | 0.1mg | EUR 494.4 |
|
Description: A monoclonal antibody from clone 25F2.D9 against Human | Mouse | Rat | Bovine | Monkey | Hamster | Sheep | Yeast | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) AHA1. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Mouse Aha1. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000). This MAb for AHA1 is conjugated with ATTO 655. |
|||
Monoclonal antibody for AHA1 |
|||
| SMC-172D-A680 | Stressmarq | 0.1mg | EUR 494.4 |
|
Description: A monoclonal antibody from clone 25F2.D9 against Human | Mouse | Rat | Bovine | Monkey | Hamster | Sheep | Yeast | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) AHA1. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Mouse Aha1. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000). This MAb for AHA1 is conjugated with ATTO 680. |
|||
Monoclonal antibody for AHA1 |
|||
| SMC-172D-A700 | Stressmarq | 0.1mg | EUR 494.4 |
|
Description: A monoclonal antibody from clone 25F2.D9 against Human | Mouse | Rat | Bovine | Monkey | Hamster | Sheep | Yeast | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) AHA1. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Mouse Aha1. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000). This MAb for AHA1 is conjugated with ATTO 700. |
|||
Monoclonal antibody for AHA1 |
|||
| SMC-172D-ALP | Stressmarq | 0.1mg | EUR 488.4 |
|
Description: A monoclonal antibody from clone 25F2.D9 against Human | Mouse | Rat | Bovine | Monkey | Hamster | Sheep | Yeast | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) AHA1. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Mouse Aha1. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000). This MAb for AHA1 is conjugated with Alkaline Phosphatase. |
|||
Monoclonal antibody for AHA1 |
|||
| SMC-172D-APC | Stressmarq | 0.1mg | EUR 493.2 |
|
Description: A monoclonal antibody from clone 25F2.D9 against Human | Mouse | Rat | Bovine | Monkey | Hamster | Sheep | Yeast | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) AHA1. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Mouse Aha1. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000). This MAb for AHA1 is conjugated with APC. |
|||
Monoclonal antibody for AHA1 |
|||
| SMC-172D-APCCY7 | Stressmarq | 0.1mg | EUR 580.8 |
|
Description: A monoclonal antibody from clone 25F2.D9 against Human | Mouse | Rat | Bovine | Monkey | Hamster | Sheep | Yeast | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) AHA1. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Mouse Aha1. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000). This MAb for AHA1 is conjugated with APC/Cy7. |
|||
Monoclonal antibody for AHA1 |
|||
| SMC-172D-BI | Stressmarq | 0.1mg | EUR 489.6 |
|
Description: A monoclonal antibody from clone 25F2.D9 against Human | Mouse | Rat | Bovine | Monkey | Hamster | Sheep | Yeast | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) AHA1. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Mouse Aha1. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000). This MAb for AHA1 is conjugated with Biotin. |
|||
Monoclonal antibody for AHA1 |
|||
| SMC-172D-DY350 | Stressmarq | 0.1mg | EUR 512.4 |
|
Description: A monoclonal antibody from clone 25F2.D9 against Human | Mouse | Rat | Bovine | Monkey | Hamster | Sheep | Yeast | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) AHA1. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Mouse Aha1. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000). This MAb for AHA1 is conjugated with Dylight 350. |
|||
Monoclonal antibody for AHA1 |
|||
| SMC-172D-DY405 | Stressmarq | 0.1mg | EUR 498 |
|
Description: A monoclonal antibody from clone 25F2.D9 against Human | Mouse | Rat | Bovine | Monkey | Hamster | Sheep | Yeast | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) AHA1. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Mouse Aha1. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000). This MAb for AHA1 is conjugated with Dylight 405. |
|||
Monoclonal antibody for AHA1 |
|||
| SMC-172D-DY488 | Stressmarq | 0.1mg | EUR 487.2 |
|
Description: A monoclonal antibody from clone 25F2.D9 against Human | Mouse | Rat | Bovine | Monkey | Hamster | Sheep | Yeast | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) AHA1. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Mouse Aha1. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000). This MAb for AHA1 is conjugated with Dylight 488. |
|||
Monoclonal antibody for AHA1 |
|||
| SMC-172D-DY594 | Stressmarq | 0.1mg | EUR 489.6 |
|
Description: A monoclonal antibody from clone 25F2.D9 against Human | Mouse | Rat | Bovine | Monkey | Hamster | Sheep | Yeast | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) AHA1. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Mouse Aha1. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000). This MAb for AHA1 is conjugated with Dylight 594. |
|||
Monoclonal antibody for AHA1 |
|||
| SMC-172D-DY633 | Stressmarq | 0.1mg | EUR 483.6 |
|
Description: A monoclonal antibody from clone 25F2.D9 against Human | Mouse | Rat | Bovine | Monkey | Hamster | Sheep | Yeast | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) AHA1. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Mouse Aha1. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000). This MAb for AHA1 is conjugated with Dylight 633. |
|||
Monoclonal antibody for AHA1 |
|||
| SMC-172D-FITC | Stressmarq | 0.1mg | EUR 486 |
|
Description: A monoclonal antibody from clone 25F2.D9 against Human | Mouse | Rat | Bovine | Monkey | Hamster | Sheep | Yeast | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) AHA1. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Mouse Aha1. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000). This MAb for AHA1 is conjugated with FITC. |
|||
Monoclonal antibody for AHA1 |
|||
| SMC-172D-HRP | Stressmarq | 0.1mg | EUR 481.2 |
|
Description: A monoclonal antibody from clone 25F2.D9 against Human | Mouse | Rat | Bovine | Monkey | Hamster | Sheep | Yeast | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) AHA1. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Mouse Aha1. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000). This MAb for AHA1 is conjugated with HRP. |
|||
Monoclonal antibody for AHA1 |
|||
| SMC-172D-P594 | Stressmarq | 0.1mg | EUR 502.8 |
|
Description: A monoclonal antibody from clone 25F2.D9 against Human | Mouse | Rat | Bovine | Monkey | Hamster | Sheep | Yeast | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) AHA1. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Mouse Aha1. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000). This MAb for AHA1 is conjugated with PE/ATTO 594. |
|||
Monoclonal antibody for AHA1 |
|||
| SMC-172D-PCP | Stressmarq | 0.1mg | EUR 493.2 |
|
Description: A monoclonal antibody from clone 25F2.D9 against Human | Mouse | Rat | Bovine | Monkey | Hamster | Sheep | Yeast | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) AHA1. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Mouse Aha1. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000). This MAb for AHA1 is conjugated with PerCP. |
|||
Monoclonal antibody for AHA1 |
|||
| SMC-172D-RPE | Stressmarq | 0.1mg | EUR 490.8 |
|
Description: A monoclonal antibody from clone 25F2.D9 against Human | Mouse | Rat | Bovine | Monkey | Hamster | Sheep | Yeast | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) AHA1. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Mouse Aha1. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000). This MAb for AHA1 is conjugated with RPE. |
|||
Monoclonal antibody for AHA1 |
|||
| SMC-172D-STR | Stressmarq | 0.1mg | EUR 492 |
|
Description: A monoclonal antibody from clone 25F2.D9 against Human | Mouse | Rat | Bovine | Monkey | Hamster | Sheep | Yeast | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) AHA1. The host species for the production of this antibody is Rat. The antigen used for immunization is Mouse Mouse Aha1. The antibody is tested and validated for WB, IHC, ICC/IF, IP, ELISA assays with the following recommended dilutions: WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000). This MAb for AHA1 is conjugated with Streptavidin. |
|||
Regenerative impact of recombinant human bone morphogenetic protein-2/absorbable collagen sponge (rhBMP-2/ACS) after sequestrectomy of medication-related osteonecrosis of the jaw (MRONJ)
Aims: Past the unique software permitted by the U.S. Meals and Drug Administration, recombinant human bone morphogenetic protein-2 (rhBMP-2) is used for medication-related osteonecrosis of the jaw (MRONJ) remedy due to its bone transforming enhancement properties. The aim of the research was to analyze the bone formation impact of rhBMP-2/absorbable collagen sponge (ACS) in sufferers with MRONJ.
Supplies and strategies: On this retrospective cohort research, 26 feminine sufferers recognized with MRONJ and who underwent mandibular sequestrectomy at Ajou College Dental Hospital from 2010 to 2018 had been included. The experimental group was composed of 18 sufferers who acquired rhBMP-2/ACSafter sequestrectomy, whereas the management group was composed of Eight sufferers who didn’t obtain rhBMP-2/ACS after sequestrectomy. A complete dose of 0.5 mg of rhBMP-2 was used within the experimental group at a focus of 0.5 mg/mL.
Observe-up panoramic X-rays had been taken instantly after the surgical procedure and greater than 6 months after the surgical procedure. Utilizing these X-rays, a radiographic index of bone defect space was calculated utilizing the modified Ihan Hren technique, which measures radiographic density of the conventional bone and the defect web site.
Outcomes: This research means that rhBMP-2 contributes to new bone formation. The imply radiographic index instantly after surgical procedure and greater than 6 months after the surgical procedure for the experimental group was 68.4% and 79.8%, respectively. The imply radiographic index instantly after surgical procedure and greater than 6 months after the surgical procedure for the management group was 73.4% and 76.7%, respectively (Wilcoxon signed rank take a look at, P>0.05). The imply radiographic index elevated 11.4% within the experimental group and three.27% within the management group (Mann-Whitney U-test, P <0.05).
Conclusion: Based mostly on the outcomes, use of rhBMP-2/ACS on bone defect websites after sequestrectomy might be a profitable technique for remedy of MRONJ sufferers.
Key phrases: Bisphosphonate-associated osteonecrosis of the jaw; Osteomyelitis; Osteonecrosis; Recombinant human bone morphogenetic protein-2.
Utility of the Leishmania infantum 21 kDa recombinant protein for the event of an immunochromatographic take a look at
Background: Visceral leishmaniasis (VL), brought on by Leishmania infantum is a systemic parasitic illness and presents a worldwide well being downside which might be deadly if not recognized and handled. Canine are the primary hosts and supply reservoirs for the transmission of the illness to people.
Strategies: On this research, the gene encoding a 21 kDa protein was cloned and expressed as a fusion protein in Escherichia coli pressure BL21 (DE3) for creating a speedy immunochromatographic take a look at (ICT) to establish contaminated canine.
The expression of the recombinant 21 kDa protein (r21) was investigated utilizing SDS-PAGE and western blotstrategies. The purified r21 kDa protein was noticed onto ICT strips and examined by sera from experimentally contaminated, naturally contaminated, and uninfected canine.
Outcomes: The SDS-PAGE and western blot strategies confirmed the profitable expression of r21 kDa protein. The ICT strip take a look at revealed that the r21 kDa protein was detected by the sera of experimentally and naturally contaminated canine. The specificity assessments additionally confirmed no cross-reactivity with animals contaminated with Trypanosoma cruzi, Toxoplasma gondii, and Ehrlichia canis.
Conclusions: Based mostly on these findings the brand new r21 kDa protein could also be an acceptable goal for creating a brand new easy, particular and speedy serological technique to detect VL in contaminated canine.
Key phrases: Leishmania infantum; 21 kDa protein; Canine; Immunochromatographic take a look at; Visceral leishmaniasis.
Human Alpha Synuclein A30P/A53T Recombinant double mutant protein |
|||
| SYN-A3053T-R | Alpha Diagnostics | 50ug | EUR 315.6 |
alpha-Synuclein Recombinant Protein |
|||
| 92-119 | ProSci | 0.05 mg | EUR 374.1 |
|
Description: Alpha-Synuclein (SNCA) is a member of the Synuclein family. SNCA is expressed principally in brain but also expressed in low concentrations in all tissues except liver. SNCA interacts with UCHL1, Phospholipase D and histones. SNCA can include beta- and gamma-synuclein. In addition, SNCA is an important regulatory component of vesicular transport in neuronal cells. It has been suggested that SNCA is related to the pathogenesis of Parkinson's Disease and neurodegenerative disorders. Defects in SNCA will lead to Dementia Lewy Body (DLB). |
|||
alpha-Synuclein Recombinant Protein |
|||
| 92-261 | ProSci | 0.05 mg | EUR 374.1 |
|
Description: Alpha-Synuclein (SNCA) is a member of the Synuclein family. SNCA is expressed principally in brain but also expressed in low concentrations in all tissues except liver. SNCA interacts with UCHL1, Phospholipase D and histones. SNCA can include beta- and gamma-synuclein. In addition, SNCA is an important regulatory component of vesicular transport in neuronal cells. It has been suggested that SNCA is related to the pathogenesis of Parkinson's Disease and neurodegenerative disorders. Defects in SNCA will lead to Dementia Lewy Body (DLB). |
|||
alpha-synuclein Recombinant Protein |
|||
| 92-520 | ProSci | 0.05 mg | EUR 739.5 |
|
Description: Alpha-synuclein (Snca) belongs to a family of proteins including a-, b-, and g-synucleins. Alpha-synuclein has been found to be implicated in the pathophysiology of many neurodegenerative diseases, including Parkinson's disease (PD) and Alzheimer's disease. Manyneurodegenerative diseases has shown that alpha-synuclein accumulates in dystrophic neurites and in Lewy bodies. The function of alpha-synuclein is closely correlated with its three-dimensional structure, especially for proteins important in the pathogenesis of neurodegenerative diseases. Alpha-synuclein is a dynamic molecule whose secondary structure depends on the environment. For example, it has an unfolded random coil structure in aqueous solution, forms a-helical structure upon binding to acidic phospholipid vesicles, and forms insoluble fibrils with a high b-sheet content that resemble the filaments found in Lewy bodies. Also, alpha-synuclein was known to associate with 14-3-3 proteins including protein kinase C, BAD, and extracellular regulated kinase, and overexpression of alpha-synuclein could contribute to cell death in neurodegenerative diseases. |
|||
Alpha-Synuclein Recombinant Protein |
|||
| 11-240 | ProSci | 0.2 mg | EUR 468.6 |
|
Description: Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca2+ release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity. |
|||
alpha-synuclein Recombinant Protein |
|||
| 92-615 | ProSci | 0.05 mg | EUR 374.1 |
|
Description: Alpha-Synuclein (SNCA) is a member of the Synuclein family. SNCA is expressed principally in brain but also expressed in low concentrations in all tissues except liver. SNCA interacts with UCHL1, Phospholipase D and histones. SNCA can include beta- and gamma-synuclein. In addition, SNCA is an important regulatory component of vesicular transport in neuronal cells. It has been suggested that SNCA is related to the pathogenesis of Parkinson's Disease and neurodegenerative disorders. Defects in SNCA will lead to Dementia Lewy Body (DLB). |
|||
Recombinant Human Alpha-Synuclein |
|||
| 7-04429 | CHI Scientific | 20µg | Ask for price |
Recombinant Human Alpha-Synuclein |
|||
| 7-04430 | CHI Scientific | 100µg | Ask for price |
Recombinant Human Alpha-Synuclein |
|||
| 7-04431 | CHI Scientific | 1mg | Ask for price |
Recombinant purified Human Alpha Synuclein mutant protein (1-60 aa) |
|||
| SYN-160-R | Alpha Diagnostics | 50ug | EUR 315.6 |
Recombinant purified Human Alpha Synuclein mutant protein (1-95 aa) |
|||
| SYN-195-R | Alpha Diagnostics | 50ug | EUR 315.6 |
Recombinant purified Human Alpha Synuclein mutant protein (61-140 aa) |
|||
| SYN-61140-R | Alpha Diagnostics | 50ug | EUR 315.6 |
Human Alpha Synuclein protein W. blot +ve control |
|||
| SYN11-C | Alpha Diagnostics | 100 ul | EUR 343.2 |
Recombinant Human Beta-Synuclein |
|||
| RP-848 | Alpha Diagnostics | 20 ug | EUR 196.8 |
Recombinant Human Gamma-Synuclein |
|||
| RP-849 | Alpha Diagnostics | 20 ug | EUR 196.8 |
SNCA Alpha Synuclein Human Recombinant Protein |
|||
| PROTP37840-8 | BosterBio | Regular: 100ug | EUR 380.4 |
|
Description: a-Synuclein Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain of 140 amino acids having a molecular mass of 14.4kDa (Real molecular weight on SDS-PAGE will be shift up). ;The Recombinant Human a-Synuclein is purified by proprietary chromatographic techniques. |
|||
Recombinant Human SNCA/Alpha-synuclein Protein |
|||
| RP00610 | Abclonal | 10 μg | EUR 151.2 |
Human Synuclein-alpha Control/blocking peptide #1 |
|||
| SYN11-P | Alpha Diagnostics | 100 ug | EUR 196.8 |
Rabbit Anti-Human Synuclein-alpha antiserum # 1 |
|||
| SYN11-S | Alpha Diagnostics | 100 ul | EUR 548.4 |
Recombinant Human Alpha-Synuclein A30P |
|||
| 7-04432 | CHI Scientific | 20µg | Ask for price |
Recombinant Human Alpha-Synuclein A30P |
|||
| 7-04433 | CHI Scientific | 100µg | Ask for price |
Recombinant Human Alpha-Synuclein A30P |
|||
| 7-04434 | CHI Scientific | 1mg | Ask for price |
Recombinant Human Alpha-Synuclein A53T |
|||
| 7-04435 | CHI Scientific | 20µg | Ask for price |
Recombinant Human Alpha-Synuclein A53T |
|||
| 7-04436 | CHI Scientific | 100µg | Ask for price |
Recombinant Human Alpha-Synuclein A53T |
|||
| 7-04437 | CHI Scientific | 1mg | Ask for price |
Recombinant Human Alpha-Synuclein NACP112 |
|||
| 7-04444 | CHI Scientific | 20µg | Ask for price |
Recombinant Human Alpha-Synuclein NACP112 |
|||
| 7-04445 | CHI Scientific | 100µg | Ask for price |
Recombinant Human Alpha-Synuclein NACP112 |
|||
| 7-04446 | CHI Scientific | 1mg | Ask for price |
alpha Synuclein protein |
|||
| 30R-1000 | Fitzgerald | 100 ug | EUR 360 |
|
Description: Purified recombinant Human alpha Synuclein protein |
|||
alpha Synuclein protein |
|||
| 30R-1001 | Fitzgerald | 100 ug | EUR 360 |
|
Description: Purified recombinant Human alpha Synuclein protein |
|||
alpha synuclein protein |
|||
| 30R-3032 | Fitzgerald | 200 ug | EUR 424.8 |
|
Description: Purified recombinant alpha synuclein protein |
|||
Recombinant purified Human Alpha Synuclein mutant protein (Delta NAC/96-140 aa) |
|||
| SYN-96140-R | Alpha Diagnostics | 50ug | EUR 315.6 |
Recombinant purified Human Alpha Synuclein protein (140 aa full length, wild type) |
|||
| SYN11-R-50 | Alpha Diagnostics | 50 ug | EUR 343.2 |
Recombinant Mouse Alpha-Synuclein |
|||
| 7-04456 | CHI Scientific | 5µg | Ask for price |
Recombinant Mouse Alpha-Synuclein |
|||
| 7-04457 | CHI Scientific | 20µg | Ask for price |
Recombinant Mouse Alpha-Synuclein |
|||
| 7-04458 | CHI Scientific | 1mg | Ask for price |
Recombinant Synuclein Alpha (SNCa) |
|||
| 4-RPB222Hu01 | Cloud-Clone |
|
|
|
Description: Recombinant Human Synuclein Alpha expressed in: E.coli |
|||
Recombinant Synuclein Alpha (SNCa) |
|||
| 4-RPB222Mu01 | Cloud-Clone |
|
|
|
Description: Recombinant Mouse Synuclein Alpha expressed in: E.coli |
|||