recombinant expressed sweet-tasting protein brazzein and its mutants in Escherichia coli

Removing of the N-terminal methionine improves the sweetness of the recombinant expressed sweet-tasting protein brazzein and its mutants in Escherichia coli

 

The sweet-tasting protein brazzein is the smallest candy protein with excessive candy efficiency. Overexpression of this protein in a heterogenous host is a vital means for its manufacturing in meals trade. On this research, the gene of minor kind brazzein was cloned into the pET-SUMO vector with optimized codon utilization and expressed in E. coli BL21-CodonPlus (DE3)-RIL.

 

The recombinant protein in absence of the N-terminal methionine displayed a sweetness threshold about 1.5 μg/ml, which is the sweetest brazzein protein reported to date. The surprising candy efficiency of the protein was validated by a sequence of mutants (7Val → Arg, 9Glu → Lys and 9Glu → Asp), wherein E9K exhibited about 50% enhancement of sweetness than the wild sort. The superior sweetness of recombinant brazzein and its sweeter mutants counsel their potential purposes in meals and drinks.

 

PRACTICAL APPLICATIONS: The sweet-tasting proteins are pure, low-, or non-caloric and nutritive, exhibiting to be promising replacers of sugars and synthetic sweeteners, and can be utilized as candy components within the fields of meals, drugs, and biotechnology. Within the current research, we report that the recombinant brazzein protein expressed in E. coli reveals superior and improved sweetness than these beforehand reported.

 

Moreover, sweeter mutants had been obtained with the expression process. The superior sweetness of recombinant brazzein and its sweeter mutants counsel their potential purposes in meals, drinks, and different fields.

 

Genoimmun
Genoimmun
Key phrases: E. coli expression; N-terminus; mutant; sweet-tasting protein brazzein; sweetness

Recombinant Yeast AHA1

MBS806931-2x01mg 2x0.1mg
EUR 675

Recombinant Yeast AHA1

MBS806931-5x005mg 5x0.05mg
EUR 1260

Recombinant Yeast AHA1

MBS806931-5x01mg 5x0.1mg
EUR 1830

Yeast Hsp90 Co-chaperone AHA1 (AHA1) Protein

abx675040-50g 50 µg
EUR 437.5

AHSA1 (AHA1, Activator of Heat Shock 90kD Protein ATPase Homolog 1 (yeast), AHA1, C14orf3, p38) (HRP)

MBS6181613-01mL 0.1(mL
EUR 875

AHSA1 (AHA1, Activator of Heat Shock 90kD Protein ATPase Homolog 1 (yeast), AHA1, C14orf3, p38) (HRP)

MBS6181613-5x01mL 5x0.1mL
EUR 3800

AHSA1 (AHA1, Activator of Heat Shock 90kD Protein ATPase Homolog 1 (yeast), AHA1, C14orf3, p38) (PE)

MBS6186023-01mL 0.1(mL
EUR 875

AHSA1 (AHA1, Activator of Heat Shock 90kD Protein ATPase Homolog 1 (yeast), AHA1, C14orf3, p38) (PE)

MBS6186023-5x01mL 5x0.1mL
EUR 3800

AHSA1 (AHA1, Activator of Heat Shock 90kD Protein ATPase Homolog 1 (yeast), AHA1, C14orf3, p38) (APC)

MBS6168383-01mL 0.1(mL
EUR 875

AHSA1 (AHA1, Activator of Heat Shock 90kD Protein ATPase Homolog 1 (yeast), AHA1, C14orf3, p38) (APC)

MBS6168383-5x01mL 5x0.1mL
EUR 3800

AHSA1 (AHA1, Activator of Heat Shock 90kD Protein ATPase Homolog 1 (yeast), AHA1, C14orf3, p38) (AP)

MBS6163973-01mL 0.1(mL
EUR 875

AHSA1 (AHA1, Activator of Heat Shock 90kD Protein ATPase Homolog 1 (yeast), AHA1, C14orf3, p38) (AP)

MBS6163973-5x01mL 5x0.1mL
EUR 3800

AHSA1 (AHA1, Activator of Heat Shock 90kD Protein ATPase Homolog 1 (yeast), AHA1, C14orf3, p38) (FITC)

MBS6177203-01mL 0.1(mL
EUR 875

AHSA1 (AHA1, Activator of Heat Shock 90kD Protein ATPase Homolog 1 (yeast), AHA1, C14orf3, p38) (FITC)

MBS6177203-5x01mL 5x0.1mL
EUR 3800

AHSA1 (AHA1, Activator of Heat Shock 90kD Protein ATPase Homolog 1 (yeast), AHA1, C14orf3, p38) (Biotin)

MBS6172793-01mL 0.1(mL
EUR 875

AHSA1 (AHA1, Activator of Heat Shock 90kD Protein ATPase Homolog 1 (yeast), AHA1, C14orf3, p38) (Biotin)

MBS6172793-5x01mL 5x0.1mL
EUR 3800

AHSA1 (AHA1, Activator of Heat Shock 90kD Protein ATPase Homolog 1 (yeast), AHA1, C14orf3, p38) (MaxLight 405)

MBS6194422-01mL 0.1(mL
EUR 875

AHSA1 (AHA1, Activator of Heat Shock 90kD Protein ATPase Homolog 1 (yeast), AHA1, C14orf3, p38) (MaxLight 405)

MBS6194422-5x01mL 5x0.1mL
EUR 3800

AHSA1 (AHA1, Activator of Heat Shock 90kD Protein ATPase Homolog 1 (yeast), AHA1, C14orf3, p38) (MaxLight 490)

MBS6205097-01mL 0.1(mL
EUR 875

AHSA1 (AHA1, Activator of Heat Shock 90kD Protein ATPase Homolog 1 (yeast), AHA1, C14orf3, p38) (MaxLight 490)

MBS6205097-5x01mL 5x0.1mL
EUR 3800

AHSA1 (AHA1, Activator of Heat Shock 90kD Protein ATPase Homolog 1 (yeast), AHA1, C14orf3, p38) (MaxLight 550)

MBS6215772-01mL 0.1(mL
EUR 875

AHSA1 (AHA1, Activator of Heat Shock 90kD Protein ATPase Homolog 1 (yeast), AHA1, C14orf3, p38) (MaxLight 550)

MBS6215772-5x01mL 5x0.1mL
EUR 3800

AHSA1 (AHA1, Activator of Heat Shock 90kD Protein ATPase Homolog 1 (yeast), AHA1, C14orf3, p38) (MaxLight 650)

MBS6226447-01mL 0.1(mL
EUR 875

AHSA1 (AHA1, Activator of Heat Shock 90kD Protein ATPase Homolog 1 (yeast), AHA1, C14orf3, p38) (MaxLight 650)

MBS6226447-5x01mL 5x0.1mL
EUR 3800

AHSA1 (AHA1, Activator of Heat Shock 90kD Protein ATPase Homolog 1 (yeast), AHA1, C14orf3, p38) (MaxLight 750)

MBS6237122-01mL 0.1(mL
EUR 875

AHSA1 (AHA1, Activator of Heat Shock 90kD Protein ATPase Homolog 1 (yeast), AHA1, C14orf3, p38) (MaxLight 750)

MBS6237122-5x01mL 5x0.1mL
EUR 3800

Aha1, Active Human Recombinant Protein

GWB-331CAB 200ug Ask for price

AHA1 (AHSA1) (NM_012111) Human Recombinant Protein

E45H41225M5 20 ug
EUR 785.42

Recombinant Yeast IDH1 Protein, Untagged, Yeast-50mg

QP10670-50mg 50mg
EUR 1251.6

Yeast TM Yeast Pst1 Recombinant Protein Liquid

IYSTTMYSTPST1R50UG each
EUR 344
Description: Yeast TM Yeast Pst1 Recombinant Protein Liquid

Yeast TM Yeast Pst1 Recombinant Protein Liquid

MBS8434691-INQUIRE INQUIRE Ask for price

GLC8 Yeast Recombinant Protein

PROTP41818 Regular: 50ug
EUR 380.4
Description: GLC8 Yeast (Saccharomyces cerevisiae) Recombinant produced in E.coli is a single, glycosylated polypeptide chain containing 265 amino acids (1-229) and having a molecular mass of 30.7 kDa (molecular size on SDS-PAGE will appear higher). ;The GLC8 is fused to a 35 amino acid His Tag at N-Terminus and purified by standard chromatography techniques.

Recombinant Yeast IDH1 Protein, Untagged, Yeast-1mg

QP10670-1mg 1mg
EUR 186

Recombinant Yeast IDH1 Protein, Untagged, Yeast-5mg

QP10670-5mg 5mg
EUR 241.2

Recombinant Saccharomyces Cerevisiae AHA1 Protein (aa 1-350) [His]

VAng-Wyb4128-1mg 1 mg
EUR 5524.8
Description: Saccharomyces Cerevisiae (strain ATCC 204508 / S288c) Aha1p protein, recombinant protein.

Yeast Yeast Kex-2 Recombinant Protein Lyophilized

IYSTYSTKEX2RLY50UG each
EUR 341
Description: Yeast Yeast Kex-2 Recombinant Protein Lyophilized

Yeast Yeast Kex-2 Recombinant Protein Lyophilized

MBS8434692-INQUIRE INQUIRE Ask for price

Ahsa1 (GFP-tagged) - Mouse AHA1, activator of heat shock protein ATPase homolog 1 (yeast) (Ahsa1)

MG205046 10 µg Ask for price

Recombinant Yeast GLRX1 Yeast Protein, His, E.coli-1mg

QP11990-1mg 1mg
EUR 1873.2

Recombinant Yeast GLRX2 Yeast Protein, His, E.coli-1mg

QP11991-1mg 1mg
EUR 1873.2

Ahsa2 (untagged) - Mouse AHA1, activator of heat shock protein ATPase homolog 2 (yeast) (Ahsa2), (10ug)

MC214260 10 µg Ask for price

Ahsa1 (untagged) - Mouse AHA1, activator of heat shock protein ATPase homolog 1 (yeast) (Ahsa1), (10ug)

MC201712 10 µg Ask for price

AHSA1 (untagged)-Human AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast) (AHSA1)

SC115514 10 µg Ask for price

AHSA2 (untagged)-Human AHA1, activator of heat shock 90kDa protein ATPase homolog 2 (yeast) (AHSA2)

SC101000 10 µg Ask for price

Recombinant Yeast GLRX1 Yeast Protein, His, E.coli-10ug

QP11990-10ug 10ug
EUR 186

Recombinant Yeast GLRX1 Yeast Protein, His, E.coli-50ug

QP11990-50ug 50ug
EUR 241.2

Recombinant Yeast GLRX2 Yeast Protein, His, E.coli-10ug

QP11991-10ug 10ug
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Recombinant Yeast GLRX2 Yeast Protein, His, E.coli-50ug

QP11991-50ug 50ug
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Recombinant Mouse AHA1

MBS806895-005mg 0.05mg
EUR 340

Recombinant Mouse AHA1

MBS806895-01mg 0.1mg
EUR 465

Recombinant Mouse AHA1

MBS806895-2x01mg 2x0.1mg
EUR 675

Recombinant Mouse AHA1

MBS806895-5x005mg 5x0.05mg
EUR 1260

Recombinant Mouse AHA1

MBS806895-5x01mg 5x0.1mg
EUR 1830

Recombinant Human AHA1

MBS806944-005mg 0.05mg
EUR 340

Recombinant Human AHA1

MBS806944-01mg 0.1mg
EUR 465

Regenerative impact of recombinant human bone morphogenetic protein-2/absorbable collagen sponge (rhBMP-2/ACS) after sequestrectomy of medication-related osteonecrosis of the jaw (MRONJ)

 

 

Aims: Past the unique software permitted by the U.S. Meals and Drug Administration, recombinant human bone morphogenetic protein-2 (rhBMP-2) is used for medication-related osteonecrosis of the jaw (MRONJ) remedy due to its bone transforming enhancement properties. The aim of the research was to analyze the bone formation impact of rhBMP-2/absorbable collagen sponge (ACS) in sufferers with MRONJ.

 

 

Supplies and strategies: On this retrospective cohort research, 26 feminine sufferers recognized with MRONJ and who underwent mandibular sequestrectomy at Ajou College Dental Hospital from 2010 to 2018 had been included. The experimental group was composed of 18 sufferers who acquired rhBMP-2/ACSafter sequestrectomy, whereas the management group was composed of Eight sufferers who didn’t obtain rhBMP-2/ACS after sequestrectomy. A complete dose of 0.5 mg of rhBMP-2 was used within the experimental group at a focus of 0.5 mg/mL.

 

Observe-up panoramic X-rays had been taken instantly after the surgical procedure and greater than 6 months after the surgical procedure. Utilizing these X-rays, a radiographic index of bone defect space was calculated utilizing the modified Ihan Hren technique, which measures radiographic density of the conventional bone and the defect web site.

 

 

Outcomes: This research means that rhBMP-2 contributes to new bone formation. The imply radiographic index instantly after surgical procedure and greater than 6 months after the surgical procedure for the experimental group was 68.4% and 79.8%, respectively. The imply radiographic index instantly after surgical procedure and greater than 6 months after the surgical procedure for the management group was 73.4% and 76.7%, respectively (Wilcoxon signed rank take a look at, P>0.05). The imply radiographic index elevated 11.4% within the experimental group and three.27% within the management group (Mann-Whitney U-test, P <0.05).

 

Conclusion: Based mostly on the outcomes, use of rhBMP-2/ACS on bone defect websites after sequestrectomy might be a profitable technique for remedy of MRONJ sufferers.

 

Key phrases: Bisphosphonate-associated osteonecrosis of the jaw; Osteomyelitis; Osteonecrosis; Recombinant human bone morphogenetic protein-2.

 

 

Utility of the Leishmania infantum 21 kDa recombinant protein for the event of an immunochromatographic take a look at

 

Recombinant purified Human Alpha Synuclein mutant protein (61-140 aa)

SYN-61140-R 50ug
EUR 315.6

Recombinant purified Human Alpha Synuclein mutant protein (1-60 aa)

SYN-160-R 50ug
EUR 315.6

Recombinant purified Human Alpha Synuclein mutant protein (1-95 aa)

SYN-195-R 50ug
EUR 315.6

Recombinant purified Human Alpha Synuclein protein (140 aa full length, wild type)

SYN11-R-50 50 ug
EUR 343.2

Recombinant purified Human Alpha Synuclein mutant protein (Delta NAC/96-140 aa)

SYN-96140-R 50ug
EUR 315.6

Recombinant (E.Coli) purified Mouse Alpha Synuclein protein (140 aa full length, wild type)

SYN12-R-20 20 ug
EUR 489.6

Human Alpha Synuclein protein W. blot +ve control

SYN11-C 100 ul
EUR 343.2

Purified recombinant Human beta Synuclein protein W. blot +ve Control

SYN13-C 100ul
EUR 343.2

Recombinant Purified Human beta Synuclein protein full length (1-134 aa)

SYN-25-R 50ug
EUR 315.6

Recombinant Purified Human Gamma Synuclein protein full length (1-127 aa)

SYN-35-R 50ug
EUR 315.6

Human Synuclein alpha Recombinant Protein

500-094 500 µg
EUR 430.5
Description: Alpha-Synuclein is a member of the Synuclein family. It is expressed principally in brain but also expressed in low concentrations in all tissues except liver. Alpha synuclein interacts with UCHL1, Phospholipase D and histones. In addition, alpha synuclein is an important regulatory component of vesicular transport in neuronal cells. It has been suggested that alpha synuclein is related to the pathogenesis of Parkinson's Disease and neurodegenerative disorders. Defects in its action will lead to Dementia Lewy Body (DLB).Human Alpha-Synuclein, is also known as Non-A Beta Component of AD Amyloid, Non-A4 Component of Amyloid Precursor, NACP, SNCA, NACP, PARK1. Recombinant Human alpha-Synuclein is produced by our E.coli expression system and the target gene encoding Met1-Ala140 is expressed, and purified as periplasmic protein by ion-exchange chromatography.

Human Synuclein alpha Recombinant Protein

500-094S 100 µg
EUR 199.5
Description: Alpha-Synuclein is a member of the Synuclein family. It is expressed principally in brain but also expressed in low concentrations in all tissues except liver. Alpha synuclein interacts with UCHL1, Phospholipase D and histones. In addition, alpha synuclein is an important regulatory component of vesicular transport in neuronal cells. It has been suggested that alpha synuclein is related to the pathogenesis of Parkinson's Disease and neurodegenerative disorders. Defects in its action will lead to Dementia Lewy Body (DLB).Human Alpha-Synuclein, is also known as Non-A Beta Component of AD Amyloid, Non-A4 Component of Amyloid Precursor, NACP, SNCA, NACP, PARK1. Recombinant Human alpha-Synuclein is produced by our E.coli expression system and the target gene encoding Met1-Ala140 is expressed, and purified as periplasmic protein by ion-exchange chromatography.

SNCA Alpha Synuclein Human Recombinant Protein

PROTP37840-8 Regular: 100ug
EUR 380.4
Description: a-Synuclein Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain of 140 amino acids having a molecular mass of 14.4kDa (Real molecular weight on SDS-PAGE will be shift up).  ;The Recombinant Human a-Synuclein is purified by proprietary chromatographic techniques.

SNCA Alpha Synuclein 61-140 Human Recombinant Protein

PROTP37840-5 Regular: 50ug
EUR 380.4
Description: A-Synuclein 61-140 Human Recombinant which is a deletion mutant of the a-synuclein amino acids 61-140 , produced in E.Coli is a single, non-glycosylated polypeptide chain of 81 amino acids having a molecular mass of 8.4kDa (molecular size on SDS-PAGE will appear higher), with an additional Met attached at the N-terminus. The Recombinant Human a-Synuclein 61-140 is purified by proprietary chromatographic techniques.

SNCA Alpha Synuclein 96-140 Human Recombinant Protein

PROTP37840-6 Regular: 50ug
EUR 380.4
Description: A-Synuclein 96-140 Human Recombinant which is a deletion mutant of the a-synuclein amino acids 96-140, produced in E.Coli is a single, non-glycosylated polypeptide chain of 46 amino acids having a molecular mass of 5.2kDa, with an additional Met attached at the N-terminus. The Recombinant Human a-Synuclein 96-140 is purified by proprietary chromatographic techniques.

alpha Synuclein (SNCA) (NM_007308) Human Recombinant Protein

PROTP37840 20ug
EUR 1249
Description: Recombinant protein of human synuclein, alpha (non A4 component of amyloid precursor) (SNCA), transcript variant NACP112

alpha Synuclein (SNCA) (NM_000345) Human Recombinant Protein

E45H32499E0 100 ug
EUR 1088.75

alpha Synuclein (SNCA) (NM_007308) Human Recombinant Protein

E45H34445M5 20 ug
EUR 785.42

alpha Synuclein (SNCA) (NM_001146055) Human Recombinant Protein

E45H33162M5 20 ug
EUR 785.42

alpha Synuclein (SNCA) (NM_001146054) Human Recombinant Protein

E45H33163M5 20 ug
EUR 785.42

alpha Synuclein (SNCA) (NM_000345) Human Recombinant Protein

E45H37189M5 20 ug
EUR 785.42

SNCA 21916 Alpha Synuclein 1-60 Human Recombinant Protein

PROTP37840-7 Regular: 50ug
EUR 380.4
Description: A-Synuclein 1-60 Human Recombinant which is a deletion mutant of the a-synuclein amino acids 1-60 and contains the N-terminal amphipathic domain, produced in E.Coli is a single, non-glycosylated polypeptide chain of 60 amino acids having a molecular mass of 6.1kDa. The Recombinant Human a-Synuclein 1-60 is purified by proprietary chromatographic techniques.

Recombinant Purified Mouse Gamma Synuclein protein full length

SYN-36-R 50ug
EUR 315.6

SNCA Alpha Synuclein NACP112 Human Recombinant Protein

PROTP37840-4 Regular: 20ug
EUR 380.4
Description: A-Synuclein NACP112 Human Recombinant which is an alternatively spliced (103-129) form of a-Synuclein, produced in E.Coli is a single, non-glycosylated polypeptide chain of 112 amino acids having a molecular mass of 11.3kDa. The Recombinant Human a-Synuclein NACP112 is purified by proprietary chromatographic techniques.

Alpha Synuclein Human Recombinant

rAP-4922 Inquiry Ask for price

SNCA Alpha Synuclein Delta-NAC Human Recombinant Protein

PROTP37840-3 Regular: 50ug
EUR 380.4
Description: A-Synuclein Delta-NAC Human Recombinant which is a deletion mutant of the a-synuclein that lacks the NAC region (amino acid 61-95), produced in E.Coli is a single, non-glycosylated polypeptide chain of 111 amino acids having a molecular mass of 11.9kDa (molecular size on SDS-PAGE will appear higher), with 6 amino acids added as a linker. The Recombinant Human a-Synuclein Delta-NAC is purified by proprietary chromatographic techniques.

recombinant human alpha synuclein

E4A10L13 100ug
EUR 595

Active Human Recombinant A53T Mutant Alpha Synuclein Protein Monomer

MBS8005531-01mg 0.1mg
EUR 490

Active Human Recombinant A53T Mutant Alpha Synuclein Protein Monomer

MBS8005531-10x01mg 10x0.1mg
EUR 2940

Active Human Recombinant A53T Mutant Alpha Synuclein Protein Monomer

MBS8005531-2x01mg 2x0.1mg
EUR 710

Active Human Recombinant A53T Mutant Alpha Synuclein Protein Monomer

MBS8005531-5x01mg 5x0.1mg
EUR 1330

Recombinant Human Synuclein‑alpha/SNCA Protein, No Tag

E40KMH1784 20ug
EUR 495

Recombinant Human Synuclein?alpha/SNCA protein, No Tag

MBS1560950-005mg 0.05mg
EUR 320

Recombinant Human Synuclein?alpha/SNCA protein, No Tag

MBS1560950-01mg 0.1mg
EUR 410

Recombinant Human Synuclein?alpha/SNCA protein, No Tag

MBS1560950-5x01mg 5x0.1mg
EUR 1520

Alpha Synuclein 61-140 Human Recombinant

rAP-4919 Inquiry Ask for price

Alpha Synuclein 96-140 Human Recombinant

rAP-4920 Inquiry Ask for price

Alpha Synuclein 1-60 Human Recombinant

rAP-4921 Inquiry Ask for price