Secretion of recombinant proteins from E. coli

The microorganism Escherichia coli is often used for recombinant protein manufacturing. Regardless of a number of advantageous traits like quick development and excessive protein yields, its lack of ability to simply secrete recombinant proteins into the extracellular medium stays a disadvantage for industrial manufacturing processes.

 

To beat this limitation, a mess of approaches to reinforce the extracellular yield and the secretion effectivity of recombinant proteins have been developed in recent times. Right here, a complete overview of secretion mechanisms for recombinant proteins from E. coli is given and divided into three principal sections.

 

First, the construction of the E. coli cell envelope and the recognized pure secretion programs are described. Second, the use and optimization of various one- or two-step secretion programs for recombinant protein manufacturing, in addition to additional permeabilization strategies are mentioned.

 

Lastly, the often-overlooked function of cell lysis in secretion research and its evaluation are addressed. To date, efficient approaches for rising the extracellular protein focus to greater than 10 g/L and virtually 100% secretion effectivity exist, nevertheless, the big vary of optimization strategies and their mixtures means that the potential for secretory protein manufacturing from E. coli has not but been totally realized.

 

Key phrases: Cell lysis; Escherichia coli; Leaky mutants; Recombinant protein manufacturing; Secretion.

 

Genoimmun
Genoimmun

Acetate formation throughout recombinant protein manufacturing in Escherichia coli Okay-12 with an elevated NAD(H) pool

 

Mouse AHA2 Protein

SPR-313C 2x0.1 mg
EUR 676.8
Description: His tagged recombinant mouse AHA2 Protein expressed in E. coli for WB, SDS-PAGE, Functional Assay. The biological activity of this protein has not yet been tested.

Recombinant Aeromonas Hydrophila AHA_3495 Protein

VAng-Lsx0947-inquire inquire Ask for price
Description: Aeromonas Hydrophila Probable intracellular septation protein A, recombinant protein.

Recombinant Aeromonas Hydrophila AHA_3669 Protein

VAng-Lsx0959-1mgEcoli 1 mg (E. coli)
EUR 4665.6
Description: Aeromonas Hydrophila tRNA1 (Val) (adenine (37)-N6)-methyltransferase, recombinant protein.

Recombinant Aeromonas Hydrophila AHA_3669 Protein

VAng-Lsx0959-500gEcoli 500 µg (E. coli)
EUR 3163.2
Description: Aeromonas Hydrophila tRNA1 (Val) (adenine (37)-N6)-methyltransferase, recombinant protein.

Recombinant Aeromonas Hydrophila AHA_3669 Protein

VAng-Lsx0959-50gEcoli 50 µg (E. coli)
EUR 2151.6
Description: Aeromonas Hydrophila tRNA1 (Val) (adenine (37)-N6)-methyltransferase, recombinant protein.

Recombinant Aeromonas Hydrophila AHA_3131 Protein

VAng-Lsx0976-1mgEcoli 1 mg (E. coli)
EUR 3949.2
Description: Aeromonas Hydrophila Putative Holliday junction resolvase, recombinant protein.

Recombinant Aeromonas Hydrophila AHA_3131 Protein

VAng-Lsx0976-500gEcoli 500 µg (E. coli)
EUR 2671.2
Description: Aeromonas Hydrophila Putative Holliday junction resolvase, recombinant protein.

Recombinant Aeromonas Hydrophila AHA_3131 Protein

VAng-Lsx0976-50gEcoli 50 µg (E. coli)
EUR 1828.8
Description: Aeromonas Hydrophila Putative Holliday junction resolvase, recombinant protein.

Recombinant Aeromonas Hydrophila AHA_2692 Protein

VAng-Lsx0981-1mgEcoli 1 mg (E. coli)
EUR 4974
Description: Aeromonas Hydrophila Putative phosphotransferase AHA_2692, recombinant protein.

Recombinant Aeromonas Hydrophila AHA_2692 Protein

VAng-Lsx0981-500gEcoli 500 µg (E. coli)
EUR 3360
Description: Aeromonas Hydrophila Putative phosphotransferase AHA_2692, recombinant protein.

Recombinant Aeromonas Hydrophila AHA_2692 Protein

VAng-Lsx0981-50gEcoli 50 µg (E. coli)
EUR 2292
Description: Aeromonas Hydrophila Putative phosphotransferase AHA_2692, recombinant protein.

Recombinant Aeromonas Hydrophila AHA_1522 Protein

VAng-Lsx0997-1mgEcoli 1 mg (E. coli)
EUR 4749.6
Description: Aeromonas Hydrophila Probable transcriptional regulatory protein AHA_1522, recombinant protein.

Recombinant Aeromonas Hydrophila AHA_1522 Protein

VAng-Lsx0997-500gEcoli 500 µg (E. coli)
EUR 3219.6
Description: Aeromonas Hydrophila Probable transcriptional regulatory protein AHA_1522, recombinant protein.

Recombinant Aeromonas Hydrophila AHA_1522 Protein

VAng-Lsx0997-50gEcoli 50 µg (E. coli)
EUR 2193.6
Description: Aeromonas Hydrophila Probable transcriptional regulatory protein AHA_1522, recombinant protein.

Recombinant Aeromonas Hydrophila AHA_3243 Protein

VAng-Lsx0823-1mgEcoli 1 mg (E. coli)
EUR 4047.6
Description: Aeromonas Hydrophila Probable rRNA maturation factor, recombinant protein.

Recombinant Aeromonas Hydrophila AHA_3243 Protein

VAng-Lsx0823-500gEcoli 500 µg (E. coli)
EUR 2742
Description: Aeromonas Hydrophila Probable rRNA maturation factor, recombinant protein.

Recombinant Aeromonas Hydrophila AHA_3243 Protein

VAng-Lsx0823-50gEcoli 50 µg (E. coli)
EUR 1870.8
Description: Aeromonas Hydrophila Probable rRNA maturation factor, recombinant protein.

Recombinant Aeromonas Hydrophila AHA_3788 Protein

VAng-Lsx0850-1mgEcoli 1 mg (E. coli)
EUR 3555.6
Description: Aeromonas Hydrophila Probable Fe (2+)-trafficking protein, recombinant protein.

Recombinant Aeromonas Hydrophila AHA_3788 Protein

VAng-Lsx0850-500gEcoli 500 µg (E. coli)
EUR 2404.8
Description: Aeromonas Hydrophila Probable Fe (2+)-trafficking protein, recombinant protein.

Recombinant Aeromonas Hydrophila AHA_3788 Protein

VAng-Lsx0850-50gEcoli 50 µg (E. coli)
EUR 1646.4
Description: Aeromonas Hydrophila Probable Fe (2+)-trafficking protein, recombinant protein.

Recombinant Aeromonas Hydrophila AHA_1344 Protein

VAng-Lsx0680-1mgEcoli 1 mg (E. coli)
EUR 4749.6
Description: Aeromonas Hydrophila Probable phosphatase AHA_1344, recombinant protein.

Recombinant Aeromonas Hydrophila AHA_1344 Protein

VAng-Lsx0680-500gEcoli 500 µg (E. coli)
EUR 3219.6
Description: Aeromonas Hydrophila Probable phosphatase AHA_1344, recombinant protein.

Recombinant Aeromonas Hydrophila AHA_1344 Protein

VAng-Lsx0680-50gEcoli 50 µg (E. coli)
EUR 2193.6
Description: Aeromonas Hydrophila Probable phosphatase AHA_1344, recombinant protein.

Recombinant Aeromonas Hydrophila AHA_2450 Protein (aa 1-561)

VAng-Lsx0802-1mgEcoli 1 mg (E. coli)
EUR 7318.8
Description: Aeromonas Hydrophila Putative transport protein AHA_2450 (aa 1-561), recombinant protein.

Recombinant Aeromonas Hydrophila AHA_3492 Protein (aa 1-553)

VAng-Lsx0805-1mgEcoli 1 mg (E. coli)
EUR 7262.4
Description: Aeromonas Hydrophila Putative transport protein AHA_3492, recombinant protein.

Recombinant Aeromonas Hydrophila AHA_0987 Protein (aa 1-114)

VAng-Lsx0690-inquire inquire Ask for price
Description: Aeromonas Hydrophila Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE (aa 1-114), recombinant protein.

Recombinant Aeromonas Hydrophila AHA_3495 Protein (aa 1-183)

VAng-Lsx0605-inquire inquire Ask for price
Description: Aeromonas Hydrophila Probable intracellular septation protein A (aa 1-183), recombinant protein.

Human AHA2 Protein

SPR-312A 0.05 mg
EUR 313.2
Description: His tagged recombinant human AHA2 Protein expressed in E. coli for WB, SDS-PAGE. The biological activity of this protein has not yet been tested.

Human AHA2 Protein

SPR-312B 0.1 mg
EUR 452.4
Description: His tagged recombinant human AHA2 Protein expressed in E. coli for WB, SDS-PAGE. The biological activity of this protein has not yet been tested.

Human AHA2 Protein

SPR-312C 2x0.1 mg
EUR 676.8
Description: His tagged recombinant human AHA2 Protein expressed in E. coli for WB, SDS-PAGE. The biological activity of this protein has not yet been tested.

Recombinant Aeromonas Hydrophila UPF0060 AHA_2410 Protein

VAng-Lsx0948-inquire inquire Ask for price
Description: Aeromonas Hydrophila UPF0060 membrane protein AHA_2410, recombinant protein.

Recombinant Aeromonas Hydrophila UPF0745 AHA_2135 Protein

VAng-Lsx0867-1mgEcoli 1 mg (E. coli)
EUR 3584.4
Description: Aeromonas Hydrophila UPF0745 protein AHA_2135, recombinant protein.

Recombinant Aeromonas Hydrophila UPF0745 AHA_2135 Protein

VAng-Lsx0867-500gEcoli 500 µg (E. coli)
EUR 2432.4
Description: Aeromonas Hydrophila UPF0745 protein AHA_2135, recombinant protein.

Recombinant Aeromonas Hydrophila UPF0745 AHA_2135 Protein

VAng-Lsx0867-50gEcoli 50 µg (E. coli)
EUR 1660.8
Description: Aeromonas Hydrophila UPF0745 protein AHA_2135, recombinant protein.

Recombinant Aeromonas Hydrophila UPF0042 AHA_3920 Protein

VAng-Lsx0905-1mgEcoli 1 mg (E. coli)
EUR 5058
Description: Aeromonas Hydrophila UPF0042 nucleotide-binding protein AHA_3920, recombinant protein.

 

Acetate formation is an obstacle in using Escherichia coli for recombinant protein manufacturing, and lots of research have targeted on optimizing fermentation processes or altering metabolism to get rid of acetate accumulation.

 

On this examine, E. coli MEC697 (MG1655 nadR nudC mazG) maintained a bigger pool of NAD(H) in comparison with the wild-type management, and likewise amassed decrease concentrations of acetate when grown in batch tradition on glucose.

 

In steady-state cultures, the elevated complete NAD(H) present in MEC697 delayed the brink dilution price for acetate formation to a development price of 0.27 h-1. Batch and fed-batch processes utilizing MEC697 have been examined for the manufacturing of β-galactosidase as a mannequin recombinant protein.

 

Fed-batch tradition of MEC697/pTrc99A-lacZ in comparison with MG1655/pTrc99A-lacZ at a development price of 0.22 h-1 confirmed solely a modest improve of protein formation. Nevertheless, 1 L batch development of MEC697/pTrc99A-lacZ resulted in 50% decrease acetate formation in comparison with MG1655/pTrc99A-lacZ and a two-fold improve in recombinant protein manufacturing.

 

Key phrases: NAD(H) pool; acetate; batch course of; chemostat; β‐galactosidase

 

 

Novel affinity chromatography technique for the environment friendly purification of recombinant binder of sperm homolog proteins

 

In mammalian species, a household of proteins named the Binder of SPerm proteins, that are expressed within the male reproductive tract, have been proven to play a job in epididymal sperm maturation and sperm capacitation. Not too long ago, one homolog from human and two homologs from mouse have been characterised.

 

In an effort to additional examine the biochemical exercise of those proteins, environment friendly purification procedures are required to isolate the proteins. Since these proteins are produced in very minute portions, we exploited the excessive capability of Escherichia coli to supply bigger portions of recombinant proteins that have been subsequently purified utilizing affinity chromatography on a diethylaminoethyl-Sephadex A-25 column.

 

Binder of sperm proteins have been proven to work together with pseudo-choline teams akin to diethylaminoethyl by way of affinity fairly than ionic interactions. The purpose of the present examine was to develop a novel technique for purifying these recombinant proteins, produced in Escherichia coli cells.

 

Diethylaminoethyl is positively charged and is a weak anion exchanger, however binder of sperm proteins work together with affinity to this resin. This examine presents a brand new, speedy, and cost-effective purification technique that gives with an distinctive purity stage, which can be utilized to review their roles in mammalian fertilization. This text is protected by copyright. All rights reserved.

 

Key phrases: Affinity purification; Binder of SPerm proteins; Diethylaminoethyl; Epididymal proteins; Ionic interactions.

Recombinant purified Human Alpha Synuclein (E46K) mutant protein

SYN-E46K-R 50ug
EUR 315.6

Human Alpha Synuclein Recombinant deletion mutant protein (96-140aa)

SD96140-R 25ug
EUR 315.6

Human Alpha Synuclein Recombinant deletion mutant protein (61-140aa)

SD61140-R 25ug
EUR 315.6

Recombinant purified Human Alpha Synuclein mutant protein (61-140 aa)

SYN-61140-R 50ug
EUR 315.6

Human Alpha Synuclein A30P/A53T Recombinant double mutant protein

SYN-A3053T-R 50ug
EUR 315.6

Recombinant purified Human Alpha Synuclein mutant protein (1-60 aa)

SYN-160-R 50ug
EUR 315.6

Recombinant purified Human Alpha Synuclein mutant protein (1-95 aa)

SYN-195-R 50ug
EUR 315.6

Recombinant purified Human Alpha Synuclein protein (140 aa full length, wild type)

SYN11-R-50 50 ug
EUR 343.2

Recombinant purified Human Alpha Synuclein mutant protein (Delta NAC/96-140 aa)

SYN-96140-R 50ug
EUR 315.6

Recombinant (E.Coli) purified Mouse Alpha Synuclein protein (140 aa full length, wild type)

SYN12-R-20 20 ug
EUR 489.6

SNCA Alpha Synuclein Human Recombinant Protein

PROTP37840-8 Regular: 100ug
EUR 380.4
Description: a-Synuclein Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain of 140 amino acids having a molecular mass of 14.4kDa (Real molecular weight on SDS-PAGE will be shift up).  ;The Recombinant Human a-Synuclein is purified by proprietary chromatographic techniques.

SNCA Alpha Synuclein 61-140 Human Recombinant Protein

PROTP37840-5 Regular: 50ug
EUR 380.4
Description: A-Synuclein 61-140 Human Recombinant which is a deletion mutant of the a-synuclein amino acids 61-140 , produced in E.Coli is a single, non-glycosylated polypeptide chain of 81 amino acids having a molecular mass of 8.4kDa (molecular size on SDS-PAGE will appear higher), with an additional Met attached at the N-terminus. The Recombinant Human a-Synuclein 61-140 is purified by proprietary chromatographic techniques.

SNCA Alpha Synuclein 96-140 Human Recombinant Protein

PROTP37840-6 Regular: 50ug
EUR 380.4
Description: A-Synuclein 96-140 Human Recombinant which is a deletion mutant of the a-synuclein amino acids 96-140, produced in E.Coli is a single, non-glycosylated polypeptide chain of 46 amino acids having a molecular mass of 5.2kDa, with an additional Met attached at the N-terminus. The Recombinant Human a-Synuclein 96-140 is purified by proprietary chromatographic techniques.

Purified recombinant Human beta Synuclein protein W. blot +ve Control

SYN13-C 100ul
EUR 343.2

Recombinant Purified Human beta Synuclein protein full length (1-134 aa)

SYN-25-R 50ug
EUR 315.6

Recombinant Purified Human Gamma Synuclein protein full length (1-127 aa)

SYN-35-R 50ug
EUR 315.6

SNCA 21916 Alpha Synuclein 1-60 Human Recombinant Protein

PROTP37840-7 Regular: 50ug
EUR 380.4
Description: A-Synuclein 1-60 Human Recombinant which is a deletion mutant of the a-synuclein amino acids 1-60 and contains the N-terminal amphipathic domain, produced in E.Coli is a single, non-glycosylated polypeptide chain of 60 amino acids having a molecular mass of 6.1kDa. The Recombinant Human a-Synuclein 1-60 is purified by proprietary chromatographic techniques.

SNCA Alpha Synuclein NACP112 Human Recombinant Protein

PROTP37840-4 Regular: 20ug
EUR 380.4
Description: A-Synuclein NACP112 Human Recombinant which is an alternatively spliced (103-129) form of a-Synuclein, produced in E.Coli is a single, non-glycosylated polypeptide chain of 112 amino acids having a molecular mass of 11.3kDa. The Recombinant Human a-Synuclein NACP112 is purified by proprietary chromatographic techniques.

Human Alpha Synuclein protein W. blot +ve control

SYN11-C 100 ul
EUR 343.2

Recombinant Purified Mouse Gamma Synuclein protein full length

SYN-36-R 50ug
EUR 315.6

SNCA Alpha Synuclein Delta-NAC Human Recombinant Protein

PROTP37840-3 Regular: 50ug
EUR 380.4
Description: A-Synuclein Delta-NAC Human Recombinant which is a deletion mutant of the a-synuclein that lacks the NAC region (amino acid 61-95), produced in E.Coli is a single, non-glycosylated polypeptide chain of 111 amino acids having a molecular mass of 11.9kDa (molecular size on SDS-PAGE will appear higher), with 6 amino acids added as a linker. The Recombinant Human a-Synuclein Delta-NAC is purified by proprietary chromatographic techniques.

Recombinant Human Synuclein‑alpha/SNCA Protein, No Tag

E40KMH1784 20ug
EUR 495

Recombinant Human Synuclein‑alpha/SNCA N-his Protein, His Tag

E40KMH1783 20ug
EUR 495

Recombinant Synuclein Alpha (SNCa)

4-RPB222Hu01
  • EUR 614.59
  • EUR 288.00
  • EUR 1974.72
  • EUR 738.24
  • EUR 1356.48
  • EUR 487.20
  • EUR 4756.80
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Synuclein Alpha expressed in: E.coli

Recombinant Synuclein Alpha (SNCa)

4-RPB222Mu01
  • EUR 614.59
  • EUR 288.00
  • EUR 1974.72
  • EUR 738.24
  • EUR 1356.48
  • EUR 487.20
  • EUR 4756.80
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Synuclein Alpha expressed in: E.coli

Recombinant Synuclein Alpha (SNCa)

4-RPB222Ra01
  • EUR 623.20
  • EUR 290.40
  • EUR 2006.98
  • EUR 748.99
  • EUR 1377.98
  • EUR 492.00
  • EUR 4837.44
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Synuclein Alpha expressed in: E.coli

Recombinant Synuclein Alpha (SNCa)

RPU56911-100ug 100ug
EUR 470.4

Recombinant Synuclein Alpha (SNCa)

RPU56911-1mg 1mg
EUR 2184

Recombinant Synuclein Alpha (SNCa)

RPU56911-50ug 50ug
EUR 385

Recombinant Synuclein Alpha (SNCa)

RPU55272-100ug 100ug
EUR 566.5

Recombinant Synuclein Alpha (SNCa)

RPU55272-1mg 1mg
EUR 2511.6

Recombinant Synuclein Alpha (SNCa)

RPU55272-50ug 50ug
EUR 455.4

Recombinant Synuclein Alpha (SNCa)

RPU43939-100ug 100ug
EUR 566.5

Recombinant Synuclein Alpha (SNCa)

RPU43939-1mg 1mg
EUR 2511.6

Recombinant Synuclein Alpha (SNCa)

RPU43939-50ug 50ug
EUR 455.4

Recombinant Synuclein Alpha (SNCa)

RPU43940-100ug 100ug
EUR 576.4

Recombinant Synuclein Alpha (SNCa)

RPU43940-1mg 1mg
EUR 2555.8

Recombinant Synuclein Alpha (SNCa)

RPU43940-50ug 50ug
EUR 463.1

Recombinant Synuclein Alpha Interacting Protein 1 (SNCaIP1)

4-RPC859Ra01
  • EUR 528.58
  • EUR 265.20
  • EUR 1652.16
  • EUR 630.72
  • EUR 1141.44
  • EUR 429.60
  • EUR 3950.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Synuclein Alpha Interacting Protein 1 expressed in: E.coli

Recombinant Synuclein Alpha Interacting Protein 1 (SNCaIP1)

RPU51198-100ug 100ug
EUR 468.6

Recombinant Synuclein Alpha Interacting Protein 1 (SNCaIP1)

RPU51198-1mg 1mg
EUR 2074.8

Recombinant Synuclein Alpha Interacting Protein 1 (SNCaIP1)

RPU51198-50ug 50ug
EUR 376.2

alpha+beta Synuclein recombinant monoclonal antibody

A5623 100ul X 3
EUR 714
Description: A recombinant monoclonal antibody from rabbit against human alpha+beta Synuclein for WB ,ELISA

Human Gamma Synuclein protein W. blot +ve control

SYN15-C 100 ul
EUR 343.2

Human alpha Synuclein 1-60 Protein

20-abx260429
  • EUR 594.00
  • EUR 276.00
  • EUR 2798.40
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 50 ug

alpha Synuclein protein

30R-1000 100 ug
EUR 360
Description: Purified recombinant Human alpha Synuclein protein

alpha Synuclein protein

30R-1001 100 ug
EUR 360
Description: Purified recombinant Human alpha Synuclein protein

alpha synuclein protein

30R-3032 200 ug
EUR 424.8
Description: Purified recombinant alpha synuclein protein

alpha Synuclein 61140 protein

30R-1007 100 ug
EUR 268.8
Description: Purified recombinant Human alpha Synuclein 61140 protein

alpha Synuclein 96140 protein

30R-1008 100 ug
EUR 268.8
Description: Purified recombinant Human alpha Synuclein 96140 protein

alpha Synuclein 160 protein

30R-1009 100 ug
EUR 268.8
Description: Purified recombinant Human alpha Synuclein 160 protein

alpha Synuclein 195 protein

30R-1013 100 ug
EUR 268.8
Description: Purified recombinant Human alpha Synuclein 195 protein

Human Synuclein Alpha (SNCa) Protein

20-abx652152
  • EUR 861.60
  • EUR 343.20
  • EUR 2665.20
  • EUR 1028.40
  • EUR 610.80
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Active Human Alpha Synuclein Protein Filaments

SPR-450B 0.1 mg
EUR 477.6
Description: Untagged recombinant human Alpha Synuclein Protein expressed in E. coli for WB, SDS-PAGE, In vivo assay, In vitro assay. This protein is biologically active.

Active Human Alpha Synuclein Protein Filaments

SPR-450C 2x0.1 mg
EUR 717.6
Description: Untagged recombinant human Alpha Synuclein Protein expressed in E. coli for WB, SDS-PAGE, In vivo assay, In vitro assay. This protein is biologically active.

Active Human Alpha Synuclein Protein Filaments

SPR-450E 5x0.1 mg
EUR 1376.4
Description: Untagged recombinant human Alpha Synuclein Protein expressed in E. coli for WB, SDS-PAGE, In vivo assay, In vitro assay. This protein is biologically active.

Recombinant Human α-Synuclein/SNCA

CF66-10ug 10ug
EUR 96
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

Recombinant Human α-Synuclein/SNCA

CF66-1mg 1mg
EUR 1216.8
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.