SequaGel

The UreaGel System consists of UreaGel Concentrate, UreaGel Diluent, and UreaGel Buffer. This system provides a convenient, dependable means for researchers to prepare gels of varying percentages. With the UreaGel System, the researcher readily prepares any commonly used gel formulation up to 20% monomer (19:1 acrylamide: bisacrylamide).

Liter bottles of UreaGel Concentrate contain 237.5 grams of acrylamide, 12.5 grams of methylene bisacrylamide, and 7.5M urea in a deionized aqueous solution. UreaGel Diluent is supplied in 450 ml and 1-liter bottles containing 7.5M urea in deionized water. UreaGel Buffer is supplied in 100 ml and 200 ml bottles containing 0.89M Tris-Borate-20mM EDTA buffer pH 8.3 (10X TBE) and 7.5M urea.

Storage: UreaGel Concentrate, UreaGel Diluent, and UreaGel Buffer are stable for one year when stored tightly capped, in a dark area at room temperature (20°C).

Ready-to-Use System for Pouring 19:1 Acrylamide: Bisacrylamide Denaturing Gels from 4% to 20% Monomer

The SequaGel UreaGel System (EC-833) contains UreaGel Concentrate, UreaGel Diluent, and UreaGel Buffer. UreaGel Concentrate contains 237.5 g/L of acrylamide, 12.5 g/L of methylene bisacrylamide, and 7.5M urea in a deionized aqueous solution. UreaGel Diluent is supplied in 450 ml and 1-liter bottles containing urea in deionized water. UreaGel Buffer is supplied in 100 ml and 200 ml bottles containing 0.89M tris-borate-20mM EDTA buffer pH 8.3 (10X TBE) and urea. Store solutions tightly capped in a dark area at room temperature (20o C). Urea may precipitate if these solutions are refrigerated. This area will redissolve when the solution returns to room temperature.

DANGER: Acrylamide has been found to be neurotoxic. Protective gloves and eyewear should be worn while handling these products. If accidental exposure occurs, contact a physician immediately.

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