Exploration of Recombinant Fusion Proteins YAPO and YAPL as Carrier Proteins for Glycoconjugate Vaccine Design against Streptococcus pneumoniae Infection

Pneumolysin (Ply), pneumococcal floor protein A (PspA), and pneumococcal floor adhesin A (PsaA) are promising cell floor protein antigen targets for Streptococcus pneumoniae (Spn) vaccine improvement. Herein, we designed and recombined two fusion proteins, named YAPO and YAPL, which contained the principle antigenic epitopes of Ply, PspA, and PsaA.

 

In-depth immunological evaluations revealed that YAPO and YAPL had robust immunocompetence to be well-qualified potential service proteins. To confirm this chance, a serotype three Spn (ST3) CPS pentasaccharide was conjugated to each fusion proteins, respectively, to generate the resultant glycoconjugates.

 

Immunological research in mice disclosed, as in contrast with TT conjugate, YAPO and YAPL conjugates provoked strong T-cell dependent immune responses that might present higher recognition, in vitro environment friendly opsonophagocytosis and in vivo efficient safety towards varied serotypes of Spn.

Genoimmun
Genoimmun

 

Collectively, YAPO and YAPL have been recognized as immunopotentiating carriers that might assist convert immunologically inactive ST3 pentasaccharide right into a T cell-dependent antigen and supply environment friendly and broad spectrum of immunoprotection protection in order to formulate purposeful glycoconjugate vaccines towards Spn infections.

 

Manufacturing of pharmaceutical energetic recombinant globular adiponectin as a secretory protein in Withania Somnifera furry root tradition

 

Amongst varied in vitro plant tradition methods, furry root methods appear to be one of the crucial interesting strategies of recombinant protein manufacturing as a consequence of their benefits in combining each whole-plant cultivation and suspension cell tradition platforms.

 

It is a report on the manufacturing and secretion of a recombinant pharmaceutically energetic protein from furry roots cultures of Withania somnifera to enhance the financial potential of this plant for the manufacturing pharmaceutical compounds.

 

 

Recombinant Human SOD2/Mn-SOD Protein (His Tag)

PKSH033072-50ug 50ug
EUR 593
Description: Human

Genorise® recombinant human mn-SOD protein

GR119158 5 µg
EUR 285

Recombinant Human SOD2/Mn-SOD Protein (His Tag, Human Cells)

PKSH033071-10ug 10ug
EUR 263
Description: Human

Recombinant Human SOD2/Mn-SOD Protein (His Tag, Human Cells)

PKSH033071-50ug 50ug
EUR 447
Description: Human

Recombinant Human SOD2/Mn-SOD (N-6His)

AP75309 1mg
EUR 1459

Recombinant Human SOD2/Mn-SOD (N-6His)

C677-10ug 10ug
EUR 242.4
Description: Supplied as a 0.2 μm filtered solution of 20mM Tris,100mM NaCl,50% glycerol,pH 8.0.

Recombinant Human SOD2/Mn-SOD (N-6His)

C677-1mg 1mg
EUR 2739.6
Description: Supplied as a 0.2 μm filtered solution of 20mM Tris,100mM NaCl,50% glycerol,pH 8.0.

Recombinant Human SOD2/Mn-SOD (N-6His)

C677-500ug 500ug
EUR 1935.6
Description: Supplied as a 0.2 μm filtered solution of 20mM Tris,100mM NaCl,50% glycerol,pH 8.0.

Recombinant Human SOD2/Mn-SOD (N-6His)

C677-50ug 50ug
EUR 595.2
Description: Supplied as a 0.2 μm filtered solution of 20mM Tris,100mM NaCl,50% glycerol,pH 8.0.

Genorise® recombinant Mouse mn-SOD protein

GR124158 5 µg
EUR 285

Recombinant Human SOD2/Mn-SOD (C-6His, Human Cells)

CB01-10ug 10ug
EUR 175.2
Description: Supplied as a 0.2 μm filtered solution of 20mM Tris,150mM NaCl,pH8.0.

Recombinant Human SOD2/Mn-SOD (C-6His, Human Cells)

CB01-1mg 1mg
EUR 2739.6
Description: Supplied as a 0.2 μm filtered solution of 20mM Tris,150mM NaCl,pH8.0.

Recombinant Human SOD2/Mn-SOD (C-6His, Human Cells)

CB01-500ug 500ug
EUR 1935.6
Description: Supplied as a 0.2 μm filtered solution of 20mM Tris,150mM NaCl,pH8.0.

Recombinant Human SOD2/Mn-SOD (C-6His, Human Cells)

CB01-50ug 50ug
EUR 363.6
Description: Supplied as a 0.2 μm filtered solution of 20mM Tris,150mM NaCl,pH8.0.

SOD Superoxide Dismutase Human Recombinant Protein

PROTP00441 Regular: 100ug
EUR 380.4
Description: Recombinant Human Cu/Zn Superoxide Dismutase produced in E.Coli is a non-glycosylated homodimeric polypeptide chain containing 2 x 153 amino acids and having a total molecular mass of 31.6kDa. 

Recombinant MARV sod (aa 1-202) Protein

VAng-Wyb3558-1mgEcoli 1 mg (E. coli)
EUR 4184.4
Description: Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) superoxide dismutase protein, recombinant protein.

Recombinant MARV sod (aa 1-202) Protein

VAng-Wyb3558-500gEcoli 500 µg (E. coli)
EUR 2997.6
Description: Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) superoxide dismutase protein, recombinant protein.

Recombinant MARV sod (aa 1-202) Protein

VAng-Wyb3558-50gEcoli 50 µg (E. coli)
EUR 2040
Description: Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) superoxide dismutase protein, recombinant protein.

Recombinant Human Cu/Zn SOD

HEENP-0301 100ug Ask for price

Mn SOD Protein

SPR-130A 0.05 mg
EUR 468
Description: Rat Recombinant Mn SOD Protein

Mn SOD Protein

SPR-130B 0.1 mg
EUR 867
Description: Rat Recombinant Mn SOD Protein

Mn SOD Protein

SPR-130C 2x0.1 mg
EUR 1561
Description: Rat Recombinant Mn SOD Protein

Mn SOD Protein

SPR-131A 0.05 mg
EUR 468
Description: Human Recombinant Mn SOD Protein

Mn SOD Protein

SPR-131B 0.1 mg
EUR 867
Description: Human Recombinant Mn SOD Protein

Mn SOD Protein

SPR-131C 2x0.1 mg
EUR 1561
Description: Human Recombinant Mn SOD Protein

Mn SOD Protein

abx675016-100g 100 µg
EUR 700

Mn SOD Protein

abx675017-100g 100 µg
EUR 700

Recombinant Human Cu/Zn SOD, His

HEENP-0302 100ug Ask for price

Superoxide Dismutase (SOD), human recombinant

4802-100 each
EUR 464.4

Superoxide Dismutase (SOD), human recombinant

4802-1000 each
EUR 2734.8

Superoxide Dismutase (SOD), human recombinant

4802-20 each
EUR 242.4

Superoxide Dismutase 2 (SOD-2), human recombinant

6360-100 each
EUR 457.2

Recombinant (Sf9) HIV-1 gp120 MN envelop protein

RP-546-10 10 ug
EUR 416.4

Recombinant Human Mn Superoxide Dismutase

13003P 1000ug
EUR 3229

Recombinant Human Mn Superoxide Dismutase

13003P-1000 1000ug
EUR 3229

 

On this examine, we chosen and synthesized a codon-optimized globular adiponectin (gAd) gene with a calreticulin sign peptide and cloned the sequence right into a plant expression binary vector containing a nptII gene as a selectable marker gene.

 

The transgenic furry roots have been produced by Agrobacterium rhizogenes-mediated transformation protocol developed by our group. Amongst ten established nptII optimistic furry roots traces, six colons considerably amassed gAd protein within the biomass and extracellular medium.

 

The presence of gAd was confirmed by western blot evaluation of root extracts. The utmost stage of furry root biomass, progress price (GR), intra- and extracellular gAd expressions have been obtained after 25 to 26 days of tradition on MS medium.

The utmost stage of intra- and extracellular gAd proteins have been discovered to be 15.19 µg/gFW and 215.7 µg/L, respectively, which resulted in a big lower within the quantity of intra- and extracellular withanolide A and withaferin A manufacturing.

The addition of PVP, KNO3 and NaCl considerably elevated the extent of extracellular gAd by roughly 13 folds. This enchancment may considerably improve the quantity of intra- and extracellular withanolide A and withaferin A manufacturing, too. The recombinant gAd produced from W. somnifera is purposeful as proved by induction the phosphorylation of ACC in C2C12 muscle cells, as its purposeful quantity was 5.1-fold greater than gAd produced from E. coli and 45% decrease than CHO cells.

Optimized high-purity protein preparation of biologically energetic recombinant VacA cytotoxin variants from Helicobacter pylori

Vacuolating cytotoxin A (VacA) is a extremely polymorphic virulence protein produced by the human gastric pathogen Helicobacter pylori which may trigger gastritis, peptic ulcer and gastric most cancers. Right here, we current an optimized protein preparation of the mature full-length VacA variants (m1-and m2-types) and their 33-kDa N-terminal and 55/59-kDa C-terminal domains as biologically energetic recombinant proteins fused with an N-terminal His(6) tag.

All recombinant VacA constructs have been over-expressed in Escherichia coli as insoluble inclusions which have been soluble when phosphate buffer (pH 7.4) was supplemented with 5-6 M urea. Upon immobilized-Ni2+ affinity purification below 5-M urea denaturing situations, homogenous merchandise (>95% purity) of 55/59-kDa domains have been constantly obtained whereas solely ∼80% purity of each mature VacA variants and the 33-kDa truncate was achieved, thus requiring further purification by size-exclusion chromatography.

After successive refolding through optimized stepwise dialysis, all refolded VacA proteins have been confirmed to own each cytotoxic and vacuolating exercise towards cultured human gastric epithelial cells albeit the exercise noticed for VacA-m2 was decrease than the m1-type variant. Such an optimized protocol described herein was efficient for manufacturing of high-purity recombinant VacA proteins in massive quantities (∼30-40 mg per liter tradition) that may pave the way in which for additional research on sequence-structure and performance relationships of various VacA variants.

 

Recombinant Human SOD2/Mn-SOD Protein (His Tag)

PKSH033072-50ug 50ug
EUR 593
Description: Human

Genorise® recombinant human mn-SOD protein

GR119158 5 µg
EUR 285

Genorise® recombinant Mouse mn-SOD protein

GR124158 5 µg
EUR 285

Recombinant Human SOD2/Mn-SOD Protein (His Tag, Human Cells)

PKSH033071-10ug 10ug
EUR 263
Description: Human

Recombinant Human SOD2/Mn-SOD Protein (His Tag, Human Cells)

PKSH033071-50ug 50ug
EUR 447
Description: Human

Recombinant Human SOD2/Mn-SOD (N-6His)

AP75309 1mg
EUR 1459

Recombinant Human SOD2/Mn-SOD (N-6His)

C677-10ug 10ug
EUR 242.4
Description: Supplied as a 0.2 μm filtered solution of 20mM Tris,100mM NaCl,50% glycerol,pH 8.0.

Recombinant Human SOD2/Mn-SOD (N-6His)

C677-1mg 1mg
EUR 2739.6
Description: Supplied as a 0.2 μm filtered solution of 20mM Tris,100mM NaCl,50% glycerol,pH 8.0.

Recombinant Human SOD2/Mn-SOD (N-6His)

C677-500ug 500ug
EUR 1935.6
Description: Supplied as a 0.2 μm filtered solution of 20mM Tris,100mM NaCl,50% glycerol,pH 8.0.

Recombinant Human SOD2/Mn-SOD (N-6His)

C677-50ug 50ug
EUR 595.2
Description: Supplied as a 0.2 μm filtered solution of 20mM Tris,100mM NaCl,50% glycerol,pH 8.0.

Recombinant MARV sod (aa 1-202) Protein

VAng-Wyb3558-1mgEcoli 1 mg (E. coli)
EUR 4184.4
Description: Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) superoxide dismutase protein, recombinant protein.

Recombinant MARV sod (aa 1-202) Protein

VAng-Wyb3558-500gEcoli 500 µg (E. coli)
EUR 2997.6
Description: Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) superoxide dismutase protein, recombinant protein.

Recombinant MARV sod (aa 1-202) Protein

VAng-Wyb3558-50gEcoli 50 µg (E. coli)
EUR 2040
Description: Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) superoxide dismutase protein, recombinant protein.

Recombinant Human SOD2/Mn-SOD (C-6His, Human Cells)

CB01-10ug 10ug
EUR 175.2
Description: Supplied as a 0.2 μm filtered solution of 20mM Tris,150mM NaCl,pH8.0.

Recombinant Human SOD2/Mn-SOD (C-6His, Human Cells)

CB01-1mg 1mg
EUR 2739.6
Description: Supplied as a 0.2 μm filtered solution of 20mM Tris,150mM NaCl,pH8.0.

Recombinant Human SOD2/Mn-SOD (C-6His, Human Cells)

CB01-500ug 500ug
EUR 1935.6
Description: Supplied as a 0.2 μm filtered solution of 20mM Tris,150mM NaCl,pH8.0.

Recombinant Human SOD2/Mn-SOD (C-6His, Human Cells)

CB01-50ug 50ug
EUR 363.6
Description: Supplied as a 0.2 μm filtered solution of 20mM Tris,150mM NaCl,pH8.0.

SOD Superoxide Dismutase Human Recombinant Protein

PROTP00441 Regular: 100ug
EUR 380.4
Description: Recombinant Human Cu/Zn Superoxide Dismutase produced in E.Coli is a non-glycosylated homodimeric polypeptide chain containing 2 x 153 amino acids and having a total molecular mass of 31.6kDa. 

Mn SOD Protein

SPR-130A 0.05 mg
EUR 468
Description: Rat Recombinant Mn SOD Protein

Mn SOD Protein

SPR-130B 0.1 mg
EUR 867
Description: Rat Recombinant Mn SOD Protein

Mn SOD Protein

SPR-130C 2x0.1 mg
EUR 1561
Description: Rat Recombinant Mn SOD Protein

Mn SOD Protein

SPR-131A 0.05 mg
EUR 468
Description: Human Recombinant Mn SOD Protein

Mn SOD Protein

SPR-131B 0.1 mg
EUR 867
Description: Human Recombinant Mn SOD Protein

Mn SOD Protein

SPR-131C 2x0.1 mg
EUR 1561
Description: Human Recombinant Mn SOD Protein

Mn SOD Protein

abx675016-100g 100 µg
EUR 700

Mn SOD Protein

abx675017-100g 100 µg
EUR 700

Recombinant (Sf9) HIV-1 gp120 MN envelop protein

RP-546-10 10 ug
EUR 416.4

SOD2 Recombinant Protein (Rat)

RP230453 100 ug Ask for price

SOD3 Recombinant Protein (Rat)

RP230456 100 ug Ask for price

Recombinant HIV-1 gp120 MN Protein, Untagged, Insect-2ug

QP12246-2ug 2ug
EUR 186

Recombinant HIV-1 gp120 MN Protein, Untagged, Insect-10ug

QP12246-10ug 10ug
EUR 316.8

Recombinant Schistosoma Mansoni EC-SOD Protein (aa 19-184)

VAng-Cr3577-1mgEcoli 1 mg (E. coli)
EUR 3921.6
Description: Schistosoma Mansoni (Blood fluke) extracellular superoxide dismutase [Cu-Zn] (EC-SOD), recombinant protein.

Recombinant Schistosoma Mansoni EC-SOD Protein (aa 19-184)

VAng-Cr3577-500gEcoli 500 µg (E. coli)
EUR 2799.6
Description: Schistosoma Mansoni (Blood fluke) extracellular superoxide dismutase [Cu-Zn] (EC-SOD), recombinant protein.

Recombinant Schistosoma Mansoni EC-SOD Protein (aa 19-184)

VAng-Cr3577-50gEcoli 50 µg (E. coli)
EUR 1924.8
Description: Schistosoma Mansoni (Blood fluke) extracellular superoxide dismutase [Cu-Zn] (EC-SOD), recombinant protein.

Recombinant HIV-1 gp120 MN Protein, Untagged, Insect-100ug

QP12246-100ug 100ug
EUR 2230.8

Recombinant Human Cu/Zn SOD

HEENP-0301 100ug Ask for price

TAGLN Recombinant Protein (Rat) (Recombinant- Tag)

RP232205 100 ug Ask for price

TAGLN2 Recombinant Protein (Rat) (Recombinant Tag)

RP232208 100 ug Ask for price

TAGLN3 Recombinant Protein (Rat) (Recombinant Tag)

RP232211 100 ug Ask for price

TAGLN3 Recombinant Protein (Rat) (Recombinant Tag)

RP232214 100 ug Ask for price

Recombinant Human Cu/Zn SOD, His

HEENP-0302 100ug Ask for price

RECOMBINANT RAT PROTEIN

RPR-100 VEGF-A / 20.6kDa MW / 5uG
EUR 195.9

RECOMBINANT RAT PROTEIN

RPR-101 VEGF-A / 20.6kDa MW / 25uG
EUR 374.72

RECOMBINANT RAT PROTEIN

RPR-102 VEGF-A / 20.6kDa MW / 2x25uG
EUR 703.9

RECOMBINANT RAT PROTEIN

RPR-110 APRIL / 16.4kDa MW / 5uG
EUR 195

RECOMBINANT RAT PROTEIN

RPR-111 APRIL / 16.4kDa MW / 25uG
EUR 374.72

RECOMBINANT RAT PROTEIN

RPR-112 APRIL / 16.4kDa MW / 2x25uG
EUR 703.9

RECOMBINANT RAT PROTEIN

RPR-113 APRIL / 16.4kDa MW / 100uG
EUR 1065.33

RECOMBINANT RAT PROTEIN

RPR-114 APRIL / 16.4kDa MW / 2x100uG
EUR 2043.61

RECOMBINANT RAT PROTEIN

RPR-115 APRIL / 16.4kDa MW / 5x100uG
EUR 4997

RECOMBINANT RAT PROTEIN

RPR-116 APRIL / 16.4kDa MW / 10x100uG
EUR 9903.14

RECOMBINANT RAT PROTEIN

RPR-120 IL-17A / 15.0kDa MW / 5uG
EUR 195

RECOMBINANT RAT PROTEIN

RPR-121 IL-17A / 15.0kDa MW / 25uG
EUR 374.72

RECOMBINANT RAT PROTEIN

RPR-122 IL-17A / 15.0kDa MW / 2x25uG
EUR 703.9

RECOMBINANT RAT PROTEIN

RPR-123 IL-17A / 15.0kDa MW / 100uG
EUR 1065.33

RECOMBINANT RAT PROTEIN

RPR-124 IL-17A / 15.0kDa MW / 2x100uG
EUR 2043.61

RECOMBINANT RAT PROTEIN

RPR-125 IL-17A / 15.0kDa MW / 5x100uG
EUR 4997

RECOMBINANT RAT PROTEIN

RPR-126 IL-17A / 15.0kDa MW / 10x10uG
EUR 9903.14

RECOMBINANT RAT PROTEIN

RPR-130 IL-13 / 13.3kDa MW / 5uG
EUR 195

RECOMBINANT RAT PROTEIN

RPR-131 IL-13 / 13.3kDa MW / 25uG
EUR 374.72

RECOMBINANT RAT PROTEIN

RPR-132 IL-13 / 13.3kDa MW / 2x25uG
EUR 703.9

RECOMBINANT RAT PROTEIN

RPR-133 IL-13 / 13.3kDa MW / 100uG
EUR 1065.33

RECOMBINANT RAT PROTEIN

RPR-135 IL-13 / 13.3kDa MW / 5x100uG
EUR 4997

RECOMBINANT RAT PROTEIN

RPR-136 IL-13 / 13.3kDa MW / 10x100uG
EUR 9903.14

Superoxide Dismutase (SOD), human recombinant

4802-100 each
EUR 464.4

Superoxide Dismutase (SOD), human recombinant

4802-1000 each
EUR 2734.8

Superoxide Dismutase (SOD), human recombinant

4802-20 each
EUR 242.4

CTAG2 Recombinant Protein (Rat) (Recombinant-P Tag)

RP196619 100 ug Ask for price

Renin, Rat recombinant protein

GWB-PS531E 100ug Ask for price

Recombinant Canine Heartworm Dirofilaria Immitis EC-SOD Protein (aa 39-195)

VAng-Cr4615-1mgEcoli 1 mg (E. coli)
EUR 3837.6
Description: Canine Heartworm Dirofilaria Immitis Extracellular superoxide dismutase [Cu-Zn] (EC-SOD), recombinant protein.

Recombinant Canine Heartworm Dirofilaria Immitis EC-SOD Protein (aa 39-195)

VAng-Cr4615-500gEcoli 500 µg (E. coli)
EUR 2749.2
Description: Canine Heartworm Dirofilaria Immitis Extracellular superoxide dismutase [Cu-Zn] (EC-SOD), recombinant protein.
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