Ganoderma lucidum, also called LINGZHI, has an extended custom of use in folks drugs of the Far East, which is documented within the oldest Chinese language pharmacopoeia, declaring it a superior drugs. LINGZHI-8 (LZ-8) is an immunoregulatory fungal protein remoted from the fruiting physique of Ganoderma lucidum. Neutropenia is a situation with an abnormally low ranges of neutrophils within the blood, which is attributable to quite a few medical situations or medicines, reminiscent of chemotherapy.
The present examine demonstrated that recombinant LZ-8 (rLZ-8) from Pichia promoted the differentiation of bone marrow hematopoietic stem cells (HSCs) into granulocytes in a neutropenia mouse mannequin induced by cyclophosphamide. Additionally, it regulated the CXCR4-SDF1 axis to advertise the mobilization of HSCs and the discharge of neutrophils from the bone marrow to peripheral blood. Binding of rLZ-Eight to the colony stimulating issue 1 receptor (CSF1R) promotes the differentiation of HSCs into primitive CFU colonies. These outcomes advised that rLZ-Eight has a possible impact within the remedy of chemotherapy-induced neutropenia.
Mixed use of recombinant human bone morphogenetic protein-7 and osteogenic media might don’t have any ultimate synergistic impact on bone regeneration of human umbilical wire mesenchymal stem cells seeded on nano-hydroxyapatite/collagen/poly (L-lactide)
Human umbilical wire mesenchymal stem cells (hUC-MSCs) are a promising different supply of mesenchymal stem cells (MSCs) which might be enormously enticing for scientific use. This examine was designed to analyze the impact of recombinant human bone morphogenetic protein-7 (rhBMP-7) and/or osteogenic media (OMD) on bone regeneration of hUC-MSCs seeded on nano-hydroxyapatite/collagen/poly (L-lactide) (nHAC/PLA) in a rabbit mannequin.
Heat Shock Protein 65, Recombinant, Mycobacterium bovis BCG (HSP65) |
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| MBS637972-5x01mg | MyBiosource | 5x0.1mg | EUR 2685 |
Recombinant Mycobacterium Bovis tal Protein (aa 1-373) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3629-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5487.6 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Transaldolase (tal), recombinant protein. |
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Recombinant Mycobacterium Bovis tal Protein (aa 1-373) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3629-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3870 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Transaldolase (tal), recombinant protein. |
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Recombinant Mycobacterium Bovis tal Protein (aa 1-373) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3629-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2650.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Transaldolase (tal), recombinant protein. |
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Recombinant Mycobacterium Bovis tam Protein (aa 1-261) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3631-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4629.6 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Trans-aconitate 2-methyltransferase (tam), recombinant protein. |
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Recombinant Mycobacterium Bovis tam Protein (aa 1-261) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3631-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3310.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Trans-aconitate 2-methyltransferase (tam), recombinant protein. |
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Recombinant Mycobacterium Bovis tam Protein (aa 1-261) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3631-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2254.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Trans-aconitate 2-methyltransferase (tam), recombinant protein. |
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Recombinant Mycobacterium Bovis tig Protein (aa 1-466) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3637-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 6196.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Trigger factor (tig), recombinant protein. |
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Recombinant Mycobacterium Bovis tig Protein (aa 1-466) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3637-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 4365.6 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Trigger factor (tig), recombinant protein. |
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Recombinant Mycobacterium Bovis tig Protein (aa 1-466) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3637-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2980.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Trigger factor (tig), recombinant protein. |
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Recombinant Mycobacterium Bovis tsf Protein (aa 1-271) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3650-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4712.4 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Elongation factor Ts (tsf), recombinant protein. |
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Recombinant Mycobacterium Bovis tsf Protein (aa 1-271) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3650-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3342 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Elongation factor Ts (tsf), recombinant protein. |
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Recombinant Mycobacterium Bovis tsf Protein (aa 1-271) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3650-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2287.2 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Elongation factor Ts (tsf), recombinant protein. |
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Recombinant Mycobacterium Bovis upp Protein (aa 1-207) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3655-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4216.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Uracil phosphoribosyltransferase (upp), recombinant protein. |
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Recombinant Mycobacterium Bovis upp Protein (aa 1-207) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3655-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3013.2 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Uracil phosphoribosyltransferase (upp), recombinant protein. |
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Recombinant Mycobacterium Bovis upp Protein (aa 1-207) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3655-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2056.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Uracil phosphoribosyltransferase (upp), recombinant protein. |
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Recombinant Mycobacterium Bovis cdh Protein (aa 1-260) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj2835-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) CDP-diacylglycerol pyrophosphatase (cdh), recombinant protein. |
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Recombinant Mycobacterium Bovis lgt Protein (aa 1-468) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj2859-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Prolipoprotein diacylglyceryl transferase (lgt), recombinant protein. |
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Recombinant Mycobacterium Bovis era Protein (aa 1-300) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj2954-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4926 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) GTPase Era (era), recombinant protein. |
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Recombinant Mycobacterium Bovis era Protein (aa 1-300) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj2954-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3490.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) GTPase Era (era), recombinant protein. |
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Recombinant Mycobacterium Bovis era Protein (aa 1-300) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj2954-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2386.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) GTPase Era (era), recombinant protein. |
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Recombinant Mycobacterium Bovis mdh Protein (aa 1-329) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3130-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5140.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Malate dehydrogenase (mdh), recombinant protein. |
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Recombinant Mycobacterium Bovis mdh Protein (aa 1-329) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3130-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3640.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Malate dehydrogenase (mdh), recombinant protein. |
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Recombinant Mycobacterium Bovis mdh Protein (aa 1-329) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3130-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2485.2 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Malate dehydrogenase (mdh), recombinant protein. |
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Recombinant Mycobacterium Bovis orn Protein (aa 1-215) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3141-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4282.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Oligoribonuclease (orn), recombinant protein. |
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Recombinant Mycobacterium Bovis orn Protein (aa 1-215) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3141-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3063.6 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Oligoribonuclease (orn), recombinant protein. |
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Recombinant Mycobacterium Bovis orn Protein (aa 1-215) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3141-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2089.2 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Oligoribonuclease (orn), recombinant protein. |
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Recombinant Mycobacterium Bovis add Protein (aa 1-365) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3211-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5421.6 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Adenosine deaminase (add), recombinant protein. |
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Recombinant Mycobacterium Bovis add Protein (aa 1-365) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3211-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3837.6 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Adenosine deaminase (add), recombinant protein. |
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Recombinant Mycobacterium Bovis add Protein (aa 1-365) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3211-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2617.2 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Adenosine deaminase (add), recombinant protein. |
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Recombinant Mycobacterium Bovis def Protein (aa 1-197) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3269-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4150.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Peptide deformylase (def), recombinant protein. |
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Recombinant Mycobacterium Bovis def Protein (aa 1-197) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3269-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2964 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Peptide deformylase (def), recombinant protein. |
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Recombinant Mycobacterium Bovis def Protein (aa 1-197) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3269-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2023.2 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Peptide deformylase (def), recombinant protein. |
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Recombinant Mycobacterium Bovis der Protein (aa 1-463) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3272-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 6164.4 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) GTPase Der (der), recombinant protein. |
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Recombinant Mycobacterium Bovis der Protein (aa 1-463) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3272-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 4348.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) GTPase Der (der), recombinant protein. |
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Recombinant Mycobacterium Bovis der Protein (aa 1-463) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3272-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2964 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) GTPase Der (der), recombinant protein. |
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Recombinant Mycobacterium Bovis efp Protein (aa 1-187) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3282-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4068 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Elongation factor P (efp), recombinant protein. |
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Recombinant Mycobacterium Bovis efp Protein (aa 1-187) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3282-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2914.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Elongation factor P (efp), recombinant protein. |
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Recombinant Mycobacterium Bovis efp Protein (aa 1-187) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3282-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1990.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Elongation factor P (efp), recombinant protein. |
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Recombinant Mycobacterium Bovis fmt Protein (aa 1-312) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3296-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5025.6 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Methionyl-tRNA formyltransferase (fmt), recombinant protein. |
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Recombinant Mycobacterium Bovis fmt Protein (aa 1-312) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3296-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3556.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Methionyl-tRNA formyltransferase (fmt), recombinant protein. |
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Recombinant Mycobacterium Bovis fmt Protein (aa 1-312) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3296-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2436 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Methionyl-tRNA formyltransferase (fmt), recombinant protein. |
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Recombinant Mycobacterium Bovis frr Protein (aa 1-185) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3302-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4052.4 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Ribosome-recycling factor (frr), recombinant protein. |
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Recombinant Mycobacterium Bovis frr Protein (aa 1-185) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3302-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2898 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Ribosome-recycling factor (frr), recombinant protein. |
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Recombinant Mycobacterium Bovis frr Protein (aa 1-185) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3302-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1974 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Ribosome-recycling factor (frr), recombinant protein. |
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Recombinant Mycobacterium Bovis mtp Protein (aa 31-103) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3476-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3228 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Pilin (mtp), recombinant protein. |
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Recombinant Mycobacterium Bovis mtp Protein (aa 31-103) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3476-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2304 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Pilin (mtp), recombinant protein. |
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Recombinant Mycobacterium Bovis mtp Protein (aa 31-103) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3476-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1594.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Pilin (mtp), recombinant protein. |
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Recombinant Mycobacterium Bovis obg Protein (aa 1-479) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3485-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 6279.6 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) GTPase obg (obg), recombinant protein. |
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Recombinant Mycobacterium Bovis obg Protein (aa 1-479) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3485-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 4448.4 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) GTPase obg (obg), recombinant protein. |
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Recombinant Mycobacterium Bovis obg Protein (aa 1-479) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3485-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 3030 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) GTPase obg (obg), recombinant protein. |
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Recombinant Mycobacterium Bovis pgk Protein (aa 1-412) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3501-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5784 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Phosphoglycerate kinase (pgk), recombinant protein. |
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Recombinant Mycobacterium Bovis pgk Protein (aa 1-412) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3501-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 4084.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Phosphoglycerate kinase (pgk), recombinant protein. |
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Recombinant Mycobacterium Bovis pgk Protein (aa 1-412) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3501-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2782.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Phosphoglycerate kinase (pgk), recombinant protein. |
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Recombinant Mycobacterium Bovis rph Protein (aa 1-259) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3556-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4612.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Ribonuclease PH (rph), recombinant protein. |
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Recombinant Mycobacterium Bovis rph Protein (aa 1-259) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3556-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3294 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Ribonuclease PH (rph), recombinant protein. |
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Recombinant Mycobacterium Bovis rph Protein (aa 1-259) (strain BCG / Pasteur 1173P2) |
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| VAng-Yyj3556-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2238 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Ribonuclease PH (rph), recombinant protein. |
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The traits of stem cells had been analyzed by plastic adherence, cell phenotype and multilineage differentiation potential. Cell proliferation was examined utilizing CCK-Eight assay. Osteogenic differentiation was evaluated by quantitative Ca2+ focus, PO43- focus, alkaline phosphatase (ALP) exercise, osteocalcin (OCN) secretion and mineralized matrix formation.
Bone regeneration was investigated in jaw bone defect restore in rabbit by micro-CT, fluorescent labeling and hematoxylin eosin staining. Aside from preliminary stress response, OMD and OMD+rhBMP-7 inhibited the proliferation of hUC-MSCs seeded on nHAC/PLA; rhBMP-7 inhibited cell proliferation within the non-logarithmic part and attenuated the inhibitory impact of OMD on cell proliferation.
The inhibitory results of OMD, rhBMP-7 and OMD+rhBMP-7 on cell proliferation had been ranked as OMD>OMD+rhBMP-7>rhBMP-7. OMD, rhBMP-7 and OMD+rhBMP-7 promoted Ca2+ focus, PO43- focus, ALP exercise, OCN secretion and mineralized matrix formation of hUC-MSCs seeded on nHAC/PLA.
The selling results of OMD, rhBMP-7 and OMD+rhBMP-7 on Ca2+ focus, PO3-Four focus, ALP exercise, OCN secretion and mineralized matrix formation had been ranked as rhBMP-7>OMD>OMD+rhBMP-7, OMD>OMD+rhBMP-7>rhBMP-7, OMD>rhBMP-7>OMD+rhBMP-7, rhBMP-7>OMD+rhBMP-7>OMD and OMD>rhBMP-7>OMD+rhBMP-7, respectively.
In rabbit jaw bone defect restore, OMD, rhBMP-7 and OMD+rhBMP-7 enhanced bone regeneration of hUC-MSCs seeded on nHAC/PLA, however the largest bone mineral apposition fee and bone formation had been offered in cultures with rhBMP-7. These findings advised that the mixed use of rhBMP-7 and OMDmight don’t have any ultimate synergistic impact on bone regeneration of hUC-MSCs seeded on nHAC/PLA in rabbit jaw bone defect.
Expression of a New Recombinant Collagenase Protein of Lucilia Sericata in SF9 Insect Cell as a Potential Methodology for Wound Therapeutic
Background: Right now, using maggot remedy has turn into widespread because of the improve in persistent ulcers on the earth. The recombinant manufacturing of secreted enzymes from these larvae is a novel non-invasive technique for the remedy of persistent ulcers. Lucilia Sericata (L. sericata) collagenase (MMP-1) has been expressed in insect cells. Collagenase is an enzyme that’s broadly utilized in scientific remedy and business.
It has been indicated that collagenase is expressed and secreted in salivary glands of L. sericata whereas utilizing for maggot debridement remedy.
Targets: Within the current examine we determined to provide the recombinant type of collagenase enzyme in Spodoptera frugiperda (SF9) insect cells utilizing the baculovirus expression system (Bac-to-Bac).
Supplies and strategies: cloned the coding sequences (residues 494-1705) of L. sericata collagenase into the pFastBacHTA as donor plasmid. After transposition within the bacmid of DH10Bac host, the bacmid was transfected into the Sf9 cell line, then the expressed recombinant collagenase (MMP-1) was purified utilizing the Ni-NTA agarose.
Outcomes: The recombinant protein was verified by Western blotting. Moreover, the organic exercise of purified protein was measured within the presence of its particular substrate and its inhibitor, which was 67 IU.mL-1 based mostly on our outcomes, it was revealed that the characterised gene in our earlier examine codes L. sericata collagenesa enzyme.
Conclusion: Contemplating to the broad purposes of collagenase in medical sciences, for the primary time, we cloned the L. sericata collagenase (MMP-1) gene into the insect cell line to ascertain a way for the expression and purification of L. sericata collagenase (MMP-1). The consequence assist for making ready and designing a protected and versatile recombinant drug in future.
Key phrases: Collagenase; MMP-1; Recombinant Protein; SF9 insect cell; Lucilia Sericata.
Recombinant Human GRP78 (Bip) |
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| MBS806942-2x01mg | MyBiosource | 2x0.1mg | EUR 650 |
Recombinant Human GRP78 (Bip) |
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| MBS806942-5x005mg | MyBiosource | 5x0.05mg | EUR 990 |
Recombinant Human GRP78 (Bip) |
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| MBS806942-5x01mg | MyBiosource | 5x0.1mg | EUR 1500 |
Recombinant Human GRP78 (Bip), native sequence Protein |
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| MBS806904-005mg | MyBiosource | 0.05mg | EUR 295 |
Recombinant Human GRP78 (Bip), native sequence Protein |
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| MBS806904-01mg | MyBiosource | 0.1mg | EUR 435 |
Recombinant Human GRP78 (Bip), native sequence Protein |
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| MBS806904-2x01mg | MyBiosource | 2x0.1mg | EUR 680 |
Recombinant Human GRP78 (Bip), native sequence Protein |
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| MBS806904-5x005mg | MyBiosource | 5x0.05mg | EUR 1065 |
Recombinant Human GRP78 (Bip), native sequence Protein |
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| MBS806904-5x01mg | MyBiosource | 5x0.1mg | EUR 1700 |
Glucose Regulated Protein 78, Recombinant, Human (Grp78, BiP, KDEL) |
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| MBS637101-005mg | MyBiosource | 0.05mg | EUR 465 |
Glucose Regulated Protein 78, Recombinant, Human (Grp78, BiP, KDEL) |
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| MBS637101-01mg | MyBiosource | 0.1mg | EUR 585 |
Glucose Regulated Protein 78, Recombinant, Human (Grp78, BiP, KDEL) |
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| MBS637101-5x01mg | MyBiosource | 5x0.1mg | EUR 2485 |
Glucose Regulated Protein 78, Recombinant, Human (Grp78, BiP, KDEL) |
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| MBS635919-005mg | MyBiosource | 0.05mg | EUR 485 |
Glucose Regulated Protein 78, Recombinant, Human (Grp78, BiP, KDEL) |
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| MBS635919-01mg | MyBiosource | 0.1mg | EUR 620 |
Glucose Regulated Protein 78, Recombinant, Human (Grp78, BiP, KDEL) |
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| MBS635919-5x01mg | MyBiosource | 5x0.1mg | EUR 2640 |
Grp78/BiP (hast), (recombinant) |
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| MBS565964-005mg | MyBiosource | 0.05mg | EUR 340 |
Grp78/BiP (hast), (recombinant) |
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| MBS565964-02mg | MyBiosource | 0.2mg | EUR 675 |
Grp78/BiP (hast), (recombinant) |
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| MBS565964-5x02mg | MyBiosource | 5x0.2mg | EUR 2980 |
GRP78 (Bip) Protein |
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| SPR-107A | Stressmarq | 0.05 mg | EUR 156 |
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Description: Active Human Recombinant GRP78 (Bip) Protein |
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GRP78 (Bip) Protein |
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| SPR-107B | Stressmarq | 0.1 mg | EUR 293 |
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Description: Active Human Recombinant GRP78 (Bip) Protein |
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GRP78 (Bip) Protein |
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| SPR-107C | Stressmarq | 2x0.1 mg | EUR 542 |
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Description: Active Human Recombinant GRP78 (Bip) Protein |
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GRP78 (Bip) Protein |
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| SPR-119A | Stressmarq | 0.05 mg | EUR 179 |
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Description: Human Recombinant GRP78 (Bip) Full Length Protein |
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GRP78 (Bip) Protein |
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| SPR-119B | Stressmarq | 0.1 mg | EUR 330 |
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Description: Human Recombinant GRP78 (Bip) Full Length Protein |
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GRP78 (Bip) Protein |
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| SPR-119C | Stressmarq | 2x0.1 mg | EUR 576 |
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Description: Human Recombinant GRP78 (Bip) Full Length Protein |
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Recombinant GRP78 BiP Monoclonal Antibody |
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| E-AB-81472-100uL | Elabscience Biotech | 100uL | EUR 260 |
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Description: Unconjugated |
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Recombinant GRP78 BiP Monoclonal Antibody |
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| E-AB-81472-50uL | Elabscience Biotech | 50uL | EUR 160 |
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Description: Unconjugated |
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Recombinant GRP78 BiP Monoclonal Antibody |
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| E-AB-81472-each | Elabscience Biotech | each | Ask for price |
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Description: Unconjugated |
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GRP78 BiP |
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| E8M1505-13 | EnoGene | 100ul | EUR 275 |
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Description: Available in various conjugation types. |
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GRP78 BiP |
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| E8M1506-2 | EnoGene | 100ul | EUR 275 |
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Description: Available in various conjugation types. |
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GRP78 BiP |
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| MBS8534599-01mL | MyBiosource | 0.1mL | EUR 345 |
GRP78 BiP |
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| MBS8534599-01mLAF405L | MyBiosource | 0.1mL(AF405L) | EUR 565 |
GRP78 BiP |
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| MBS8534599-01mLAF405S | MyBiosource | 0.1mL(AF405S) | EUR 565 |
GRP78 BiP |
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| MBS8534599-01mLAF610 | MyBiosource | 0.1mL(AF610) | EUR 565 |
GRP78 BiP |
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| MBS8534599-01mLAF635 | MyBiosource | 0.1mL(AF635) | EUR 565 |
GRP78 BiP |
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| MBS8534609-01mL | MyBiosource | 0.1mL | EUR 345 |
GRP78 BiP |
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| MBS8534609-01mLAF405L | MyBiosource | 0.1mL(AF405L) | EUR 565 |
GRP78 BiP |
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| MBS8534609-01mLAF405S | MyBiosource | 0.1mL(AF405S) | EUR 565 |
GRP78 BiP |
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| MBS8534609-01mLAF610 | MyBiosource | 0.1mL(AF610) | EUR 565 |
GRP78 BiP |
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| MBS8534609-01mLAF635 | MyBiosource | 0.1mL(AF635) | EUR 565 |
Antibody for Human GRP78 (Bip) |
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| SPC-180D-A565 | Stressmarq | 0.1mg | EUR 447.6 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to ATTO 565. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-A633 | Stressmarq | 0.1mg | EUR 447.6 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to ATTO 633. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-A655 | Stressmarq | 0.1mg | EUR 447.6 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to ATTO 655. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-A680 | Stressmarq | 0.1mg | EUR 447.6 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to ATTO 680. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-A700 | Stressmarq | 0.1mg | EUR 447.6 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to ATTO 700. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-APCCY7 | Stressmarq | 0.1mg | EUR 534 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to APC/Cy7. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-DY350 | Stressmarq | 0.1mg | EUR 465.6 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to Dylight 350. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-DY405 | Stressmarq | 0.1mg | EUR 451.2 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to Dylight 405. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-DY488 | Stressmarq | 0.1mg | EUR 439.2 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to Dylight 488. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-DY594 | Stressmarq | 0.1mg | EUR 441.6 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to Dylight 594. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-DY633 | Stressmarq | 0.1mg | EUR 435.6 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to Dylight 633. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-P594 | Stressmarq | 0.1mg | EUR 456 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to PE/ATTO 594. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-STR | Stressmarq | 0.1mg | EUR 445.2 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to Streptavidin. |
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Recombinant Human GRP78 protein (His tag) |
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| PDEH100182-100ug | Elabscience Biotech | 100ug | EUR 118 |
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Description: Human |
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Recombinant Human GRP78 protein (His tag) |
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| PDEH100182-20ug | Elabscience Biotech | 20ug | EUR 68 |
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Description: Human |
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Recombinant Human GRP78 protein (His tag) |
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| MBS2570782-002mg | MyBiosource | 0.02mg | EUR 135 |
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