Ganoderma lucidum, also called LINGZHI, has an extended custom of use in folks drugs of the Far East, which is documented within the oldest Chinese language pharmacopoeia, declaring it a superior drugs. LINGZHI-8 (LZ-8) is an immunoregulatory fungal protein remoted from the fruiting physique of Ganoderma lucidum. Neutropenia is a situation with an abnormally low ranges of neutrophils within the blood, which is attributable to quite a few medical situations or medicines, reminiscent of chemotherapy.
The present examine demonstrated that recombinant LZ-8 (rLZ-8) from Pichia promoted the differentiation of bone marrow hematopoietic stem cells (HSCs) into granulocytes in a neutropenia mouse mannequin induced by cyclophosphamide. Additionally, it regulated the CXCR4-SDF1 axis to advertise the mobilization of HSCs and the discharge of neutrophils from the bone marrow to peripheral blood. Binding of rLZ-Eight to the colony stimulating issue 1 receptor (CSF1R) promotes the differentiation of HSCs into primitive CFU colonies. These outcomes advised that rLZ-Eight has a possible impact within the remedy of chemotherapy-induced neutropenia.
Mixed use of recombinant human bone morphogenetic protein-7 and osteogenic media might don’t have any ultimate synergistic impact on bone regeneration of human umbilical wire mesenchymal stem cells seeded on nano-hydroxyapatite/collagen/poly (L-lactide)
Human umbilical wire mesenchymal stem cells (hUC-MSCs) are a promising different supply of mesenchymal stem cells (MSCs) which might be enormously enticing for scientific use. This examine was designed to analyze the impact of recombinant human bone morphogenetic protein-7 (rhBMP-7) and/or osteogenic media (OMD) on bone regeneration of hUC-MSCs seeded on nano-hydroxyapatite/collagen/poly (L-lactide) (nHAC/PLA) in a rabbit mannequin.
Mycobacterium bovis BCG HSP65 Protein |
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| SPR-116C | Stressmarq | 2x0.1 mg | EUR 754.8 |
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Description: Untagged recombinant Mycobacterium bovis BCG HSP65 Protein expressed in E. coli for WB, SDS-PAGE, Functional Assay, ELISA. The biological activity of this protein has not yet been tested. |
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Recombinant Mycobacterium Bovis hspX Protein (aa 2-144) |
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| VAng-Yyj2851-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5984.4 |
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Description: Mycobacterium Bovis 14 kDa antigen (hspX), recombinant protein. |
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Recombinant Mycobacterium Bovis hspX Protein (aa 2-144) |
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| VAng-Yyj2851-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3836.4 |
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Description: Mycobacterium Bovis 14 kDa antigen (hspX), recombinant protein. |
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Recombinant Mycobacterium Bovis hspX Protein (aa 2-144) |
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| VAng-Yyj2851-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1210.8 |
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Description: Mycobacterium Bovis 14 kDa antigen (hspX), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_1543 Protein (aa 1-335) |
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| VAng-Yyj2832-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) UPF0353 protein BCG_1543 (BCG_1543), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_1434 Protein (aa 1-327) |
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| VAng-Yyj2932-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5140.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Glycolipid sulfotransferase BCG_1434 (BCG_1434), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_1434 Protein (aa 1-327) |
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| VAng-Yyj2932-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3640.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Glycolipid sulfotransferase BCG_1434 (BCG_1434), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_1434 Protein (aa 1-327) |
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| VAng-Yyj2932-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2485.2 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Glycolipid sulfotransferase BCG_1434 (BCG_1434), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2993 Protein (aa 1-282) |
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| VAng-Yyj2933-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4794 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Uncharacterized oxidoreductase BCG_2993 (BCG_2993), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2993 Protein (aa 1-282) |
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| VAng-Yyj2933-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3393.6 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Uncharacterized oxidoreductase BCG_2993 (BCG_2993), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2993 Protein (aa 1-282) |
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| VAng-Yyj2933-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2320.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Uncharacterized oxidoreductase BCG_2993 (BCG_2993), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_0069 Protein (aa 1-202) |
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| VAng-Yyj3232-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4184.4 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) UPF0301 protein BCG_0069 (BCG_0069), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_0069 Protein (aa 1-202) |
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| VAng-Yyj3232-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2997.6 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) UPF0301 protein BCG_0069 (BCG_0069), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_0069 Protein (aa 1-202) |
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| VAng-Yyj3232-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2040 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) UPF0301 protein BCG_0069 (BCG_0069), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_0095 Protein (aa 1-979) |
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| VAng-Yyj3233-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 10485.6 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) UPF0182 protein BCG_0095 (BCG_0095), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_1940 Protein (aa 1-430) |
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| VAng-Yyj3234-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5916 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) CinA-like protein (BCG_1940), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_1940 Protein (aa 1-430) |
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| VAng-Yyj3234-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 4184.4 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) CinA-like protein (BCG_1940), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_1940 Protein (aa 1-430) |
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| VAng-Yyj3234-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2848.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) CinA-like protein (BCG_1940), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2973 Protein (aa 1-270) |
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| VAng-Yyj3237-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4695.6 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Phthiotriol/phenolphthiotriol dimycocerosates methyltransferase (BCG_2973), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2973 Protein (aa 1-270) |
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| VAng-Yyj3237-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3327.6 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Phthiotriol/phenolphthiotriol dimycocerosates methyltransferase (BCG_2973), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2973 Protein (aa 1-270) |
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| VAng-Yyj3237-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2287.2 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Phthiotriol/phenolphthiotriol dimycocerosates methyltransferase (BCG_2973), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2974 Protein (aa 1-418) |
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| VAng-Yyj3238-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5817.6 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Trans-acting enoyl reductase (BCG_2974), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2974 Protein (aa 1-418) |
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| VAng-Yyj3238-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 4118.4 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Trans-acting enoyl reductase (BCG_2974), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2974 Protein (aa 1-418) |
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| VAng-Yyj3238-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2815.2 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Trans-acting enoyl reductase (BCG_2974), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2978 Protein (aa 1-256) |
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| VAng-Yyj3239-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4596 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) PGL/p-HBAD biosynthesis glycosyltransferase BCG_2978 (BCG_2978), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2978 Protein (aa 1-256) |
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| VAng-Yyj3239-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3276 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) PGL/p-HBAD biosynthesis glycosyltransferase BCG_2978 (BCG_2978), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2978 Protein (aa 1-256) |
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| VAng-Yyj3239-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2238 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) PGL/p-HBAD biosynthesis glycosyltransferase BCG_2978 (BCG_2978), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_3311 Protein (aa 1-222) |
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| VAng-Yyj3244-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4332 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Maf-like protein BCG_3311 (BCG_3311), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_3311 Protein (aa 1-222) |
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| VAng-Yyj3244-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3096 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Maf-like protein BCG_3311 (BCG_3311), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_3311 Protein (aa 1-222) |
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| VAng-Yyj3244-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2106 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Maf-like protein BCG_3311 (BCG_3311), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2153c Protein (aa 1-276) |
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| VAng-Yyj2833-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Undecaprenyl-diphosphatase (uppP), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2666c Protein (aa 1-110) |
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| VAng-Yyj2834-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) UPF0060 membrane protein BCG_2666c (BCG_2666c), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_0261c Protein (aa 1-254) |
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| VAng-Yyj2930-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4580.4 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Uncharacterized methyltransferase BCG_0261c (BCG_0261c), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_0261c Protein (aa 1-254) |
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| VAng-Yyj2930-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3261.6 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Uncharacterized methyltransferase BCG_0261c (BCG_0261c), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_0261c Protein (aa 1-254) |
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| VAng-Yyj2930-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2221.2 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Uncharacterized methyltransferase BCG_0261c (BCG_0261c), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_1280c Protein (aa 1-215) |
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| VAng-Yyj2931-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4282.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Putative O-methyltransferase BCG_1280c (BCG_1280c), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_1280c Protein (aa 1-215) |
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| VAng-Yyj2931-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3063.6 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Putative O-methyltransferase BCG_1280c (BCG_1280c), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_1280c Protein (aa 1-215) |
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| VAng-Yyj2931-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2089.2 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Putative O-methyltransferase BCG_1280c (BCG_1280c), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2919c Protein (aa 1-128) |
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| VAng-Yyj3235-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 3622.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) UPF0102 protein BCG_2919c (BCG_2919c), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2919c Protein (aa 1-128) |
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| VAng-Yyj3235-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 2601.6 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) UPF0102 protein BCG_2919c (BCG_2919c), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2919c Protein (aa 1-128) |
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| VAng-Yyj3235-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 1792.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) UPF0102 protein BCG_2919c (BCG_2919c), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2972c Protein (aa 1-381) |
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| VAng-Yyj3236-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5536.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Phthiodiolone/phenolphthiodiolone dimycocerosates ketoreductase (BCG_2972c), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2972c Protein (aa 1-381) |
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| VAng-Yyj3236-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3921.6 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Phthiodiolone/phenolphthiodiolone dimycocerosates ketoreductase (BCG_2972c), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2972c Protein (aa 1-381) |
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| VAng-Yyj3236-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2683.2 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Phthiodiolone/phenolphthiodiolone dimycocerosates ketoreductase (BCG_2972c), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2979c Protein (aa 1-366) |
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| VAng-Yyj3240-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5421.6 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Inactive PGL/p-HBAD biosynthesis glycosyltransferase BCG_2979c (BCG_2979c), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2979c Protein (aa 1-366) |
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| VAng-Yyj3240-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3837.6 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Inactive PGL/p-HBAD biosynthesis glycosyltransferase BCG_2979c (BCG_2979c), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2979c Protein (aa 1-366) |
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| VAng-Yyj3240-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2617.2 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Inactive PGL/p-HBAD biosynthesis glycosyltransferase BCG_2979c (BCG_2979c), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2980c Protein (aa 39-245) |
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| VAng-Yyj3241-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4216.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Rhamnosyl O-methyltransferase (BCG_2980c), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2980c Protein (aa 39-245) |
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| VAng-Yyj3241-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3013.2 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Rhamnosyl O-methyltransferase (BCG_2980c), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2980c Protein (aa 39-245) |
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| VAng-Yyj3241-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2056.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Rhamnosyl O-methyltransferase (BCG_2980c), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2983c Protein (aa 1-449) |
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| VAng-Yyj3242-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 6064.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) PGL/p-HBAD biosynthesis rhamnosyltransferase (BCG_2983c), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2983c Protein (aa 1-449) |
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| VAng-Yyj3242-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 4282.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) PGL/p-HBAD biosynthesis rhamnosyltransferase (BCG_2983c), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_2983c Protein (aa 1-449) |
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| VAng-Yyj3242-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2914.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) PGL/p-HBAD biosynthesis rhamnosyltransferase (BCG_2983c), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_3215c Protein (aa 1-992) |
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| VAng-Yyj3243-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 10585.2 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) UPF0182 protein BCG_3215c (BCG_3215c), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_3599c Protein (aa 1-303) |
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| VAng-Yyj3245-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4959.6 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Acetaldehyde dehydrogenase (BCG_3599c), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_3599c Protein (aa 1-303) |
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| VAng-Yyj3245-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3508.8 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Acetaldehyde dehydrogenase (BCG_3599c), recombinant protein. |
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Recombinant Mycobacterium Bovis BCG_3599c Protein (aa 1-303) |
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| VAng-Yyj3245-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2403.6 |
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Description: Mycobacterium Bovis (strain BCG / Pasteur 1173P2) Acetaldehyde dehydrogenase (BCG_3599c), recombinant protein. |
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The traits of stem cells had been analyzed by plastic adherence, cell phenotype and multilineage differentiation potential. Cell proliferation was examined utilizing CCK-Eight assay. Osteogenic differentiation was evaluated by quantitative Ca2+ focus, PO43- focus, alkaline phosphatase (ALP) exercise, osteocalcin (OCN) secretion and mineralized matrix formation.
Bone regeneration was investigated in jaw bone defect restore in rabbit by micro-CT, fluorescent labeling and hematoxylin eosin staining. Aside from preliminary stress response, OMD and OMD+rhBMP-7 inhibited the proliferation of hUC-MSCs seeded on nHAC/PLA; rhBMP-7 inhibited cell proliferation within the non-logarithmic part and attenuated the inhibitory impact of OMD on cell proliferation.
The inhibitory results of OMD, rhBMP-7 and OMD+rhBMP-7 on cell proliferation had been ranked as OMD>OMD+rhBMP-7>rhBMP-7. OMD, rhBMP-7 and OMD+rhBMP-7 promoted Ca2+ focus, PO43- focus, ALP exercise, OCN secretion and mineralized matrix formation of hUC-MSCs seeded on nHAC/PLA.
The selling results of OMD, rhBMP-7 and OMD+rhBMP-7 on Ca2+ focus, PO3-Four focus, ALP exercise, OCN secretion and mineralized matrix formation had been ranked as rhBMP-7>OMD>OMD+rhBMP-7, OMD>OMD+rhBMP-7>rhBMP-7, OMD>rhBMP-7>OMD+rhBMP-7, rhBMP-7>OMD+rhBMP-7>OMD and OMD>rhBMP-7>OMD+rhBMP-7, respectively.
In rabbit jaw bone defect restore, OMD, rhBMP-7 and OMD+rhBMP-7 enhanced bone regeneration of hUC-MSCs seeded on nHAC/PLA, however the largest bone mineral apposition fee and bone formation had been offered in cultures with rhBMP-7. These findings advised that the mixed use of rhBMP-7 and OMDmight don’t have any ultimate synergistic impact on bone regeneration of hUC-MSCs seeded on nHAC/PLA in rabbit jaw bone defect.
Expression of a New Recombinant Collagenase Protein of Lucilia Sericata in SF9 Insect Cell as a Potential Methodology for Wound Therapeutic
Background: Right now, using maggot remedy has turn into widespread because of the improve in persistent ulcers on the earth. The recombinant manufacturing of secreted enzymes from these larvae is a novel non-invasive technique for the remedy of persistent ulcers. Lucilia Sericata (L. sericata) collagenase (MMP-1) has been expressed in insect cells. Collagenase is an enzyme that’s broadly utilized in scientific remedy and business.
It has been indicated that collagenase is expressed and secreted in salivary glands of L. sericata whereas utilizing for maggot debridement remedy.
Targets: Within the current examine we determined to provide the recombinant type of collagenase enzyme in Spodoptera frugiperda (SF9) insect cells utilizing the baculovirus expression system (Bac-to-Bac).
Supplies and strategies: cloned the coding sequences (residues 494-1705) of L. sericata collagenase into the pFastBacHTA as donor plasmid. After transposition within the bacmid of DH10Bac host, the bacmid was transfected into the Sf9 cell line, then the expressed recombinant collagenase (MMP-1) was purified utilizing the Ni-NTA agarose.
Outcomes: The recombinant protein was verified by Western blotting. Moreover, the organic exercise of purified protein was measured within the presence of its particular substrate and its inhibitor, which was 67 IU.mL-1 based mostly on our outcomes, it was revealed that the characterised gene in our earlier examine codes L. sericata collagenesa enzyme.
Conclusion: Contemplating to the broad purposes of collagenase in medical sciences, for the primary time, we cloned the L. sericata collagenase (MMP-1) gene into the insect cell line to ascertain a way for the expression and purification of L. sericata collagenase (MMP-1). The consequence assist for making ready and designing a protected and versatile recombinant drug in future.
Key phrases: Collagenase; MMP-1; Recombinant Protein; SF9 insect cell; Lucilia Sericata.
Human GRP78 (Bip) Protein |
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| SPR-119C | Stressmarq | 2x0.1 mg | EUR 681.6 |
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Description: Untagged recombinant human GRP78 (Bip) Protein expressed in E. coli for WB, SDS-PAGE, ATPase Activity Assay, Functional Assay, ELISA. The biological activity of this protein has not yet been tested. |
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Active Human GRP78 (Bip) Protein |
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| SPR-107A | Stressmarq | 0.05 mg | EUR 241.2 |
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Description: His tagged recombinant human GRP78 (Bip) Protein expressed in E. coli for WB, SDS-PAGE, ATPase Activity Assay, Functional Assay, ELISA. This protein is biologically active (ATPase active). |
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Active Human GRP78 (Bip) Protein |
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| SPR-107B | Stressmarq | 0.1 mg | EUR 384 |
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Description: His tagged recombinant human GRP78 (Bip) Protein expressed in E. coli for WB, SDS-PAGE, ATPase Activity Assay, Functional Assay, ELISA. This protein is biologically active (ATPase active). |
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Active Human GRP78 (Bip) Protein |
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| SPR-107C | Stressmarq | 2x0.1 mg | EUR 645.6 |
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Description: His tagged recombinant human GRP78 (Bip) Protein expressed in E. coli for WB, SDS-PAGE, ATPase Activity Assay, Functional Assay, ELISA. This protein is biologically active (ATPase active). |
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Antibody for Human GRP78 (Bip) |
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| SPC-180C | Stressmarq | 0.025mg | EUR 216 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is unconjugated. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D | Stressmarq | 0.1mg | EUR 392.4 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is unconjugated. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-A390 | Stressmarq | 0.1mg | EUR 448.8 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to ATTO 390. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-A488 | Stressmarq | 0.1mg | EUR 447.6 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to ATTO 488. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-A565 | Stressmarq | 0.1mg | EUR 447.6 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to ATTO 565. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-A594 | Stressmarq | 0.1mg | EUR 447.6 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to ATTO 594. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-A633 | Stressmarq | 0.1mg | EUR 447.6 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to ATTO 633. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-A655 | Stressmarq | 0.1mg | EUR 447.6 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to ATTO 655. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-A680 | Stressmarq | 0.1mg | EUR 447.6 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to ATTO 680. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-A700 | Stressmarq | 0.1mg | EUR 447.6 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to ATTO 700. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-ALP | Stressmarq | 0.1mg | EUR 440.4 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to Alkaline Phosphatase. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-APC | Stressmarq | 0.1mg | EUR 446.4 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to APC . |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-APCCY7 | Stressmarq | 0.1mg | EUR 534 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to APC/Cy7. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-BI | Stressmarq | 0.1mg | EUR 442.8 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to Biotin. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-DY350 | Stressmarq | 0.1mg | EUR 465.6 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to Dylight 350. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-DY405 | Stressmarq | 0.1mg | EUR 451.2 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to Dylight 405. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-DY488 | Stressmarq | 0.1mg | EUR 439.2 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to Dylight 488. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-DY594 | Stressmarq | 0.1mg | EUR 441.6 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to Dylight 594. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-DY633 | Stressmarq | 0.1mg | EUR 435.6 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to Dylight 633. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-FITC | Stressmarq | 0.1mg | EUR 438 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to FITC. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-HRP | Stressmarq | 0.1mg | EUR 433.2 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to HRP. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-P594 | Stressmarq | 0.1mg | EUR 456 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to PE/ATTO 594. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-PCP | Stressmarq | 0.1mg | EUR 446.4 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to PerCP. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-RPE | Stressmarq | 0.1mg | EUR 444 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to RPE . |
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Antibody for Human GRP78 (Bip) |
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| SPC-180D-STR | Stressmarq | 0.1mg | EUR 445.2 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is conjugated to Streptavidin. |
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Antibody for Human GRP78 (Bip) |
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| SPC-180S | Stressmarq | 0.012mg | EUR 78 |
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Description: A polyclonal antibody for GRP78 from Human | Mouse | Rat | Dog | Fruit Fly (Drosophila melanogaster) | Hamster. The antibody is produced in rabbit after immunization with Human Full length human GRP78 (Bip) his tagged at the N terminus. The Antibody is tested and validated for WB, IHC, ICC/IF, ELISA assays with the following recommended dilutions: WB (1:2000), ICC/IF (1:100). This GRP78 antibody is unconjugated. |
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GRP78 / BIP Antibody |
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| abx233662-100ug | Abbexa | 100 ug | EUR 610.8 |
GRP78 Bip Antibody |
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| 39220-100ul | SAB | 100ul | EUR 468 |
GRP78 BiP Antibody |
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| abx038413-100ug | Abbexa | 100 ug | EUR 469.2 |
GRP78 BiP Antibody |
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| 48115-100ul | SAB | 100ul | EUR 399.6 |
GRP78 BiP Antibody |
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| 48115-50ul | SAB | 50ul | EUR 286.8 |
GRP78 BiP Antibody |
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| 48119-100ul | SAB | 100ul | EUR 399.6 |
GRP78 BiP Antibody |
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| 48119-50ul | SAB | 50ul | EUR 286.8 |
GRP78 Antibody / BiP |
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| F54005-0.1ML | NSJ Bioreagents | 0.1 ml | EUR 379 |
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Description: Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate. |
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GRP78 Antibody (BiP) |
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| R36415-100UG | NSJ Bioreagents | 100 ug | EUR 339.15 |
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Description: Additional name(s) for this target protein: HSPA5; Heat shock 70kDa protein 5; glucose-regulated protein 78 |
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GRP78 Antibody (BiP) |
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| R36416-100UG | NSJ Bioreagents | 100 ug | EUR 339.15 |
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Description: Additional name(s) for this target protein: HSPA5; Heat shock 70kDa protein 5; glucose-regulated protein 78 |
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BiP Antibody GRP78 |
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| R30913 | NSJ Bioreagents | 100 ug | EUR 356.15 |
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Description: HSPA5 (heat shock 70kDa protein 5) also known as glucose-regulated protein, 78kD(GRP78) or BiP, is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum.BiP is an essential component of the translocation machinery, as well as playing a role in retrograde transport across the ER membrane of aberrant proteins destined for degradation by theproteasome. Shen et al.(2002)concluded thatBiPretains ATF6 in the ER by inhibiting its Golgi localization signals and that dissociation ofBiPduring ER stress allows ATF6 to be transported to the Golgi. The findings ofShen et al.(2002)demonstrated thatthe proteinis a key element in sensing the folding capacity within the ER. |
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GRP78 Antibody / BiP |
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| R31939 | NSJ Bioreagents | 100 ug | EUR 356.15 |
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Description: HSPA5 (heat shock 70kDa protein 5), also known as glucose-regulated protein, 78kD (GRP78) or BiP, is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum. BiP is also an essential component of the translocation machinery, as well as playing a role in retrograde transport across the ER membrane of aberrant proteins destined for degradation by the proteasome. The HSPA5 gene is mapped on 9q33.3. Shen et al. (2002) concluded that HSPA5 retains ATF6 in the ER by inhibiting its Golgi localization signals and that dissociation of HSPA5 during ER stress allows ATF6 to be transported to the Golgi. The findings of Shen et al. (2002) demonstrated that HSPA5 is a key element in sensing the folding capacity within the ER. |
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Recombinant Echinococcus Granulosus GRP78 Protein (aa 21-651) |
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| VAng-Ly4006-inquire | Creative Biolabs | inquire | Ask for price |
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Description: Echinococcus Granulosus 78 kDa glucose-regulated protein (GRP78), partial, recombination protein. |
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GRP78 / BiP Rabbit pAb |
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| A11366-100ul | Abclonal | 100 ul | EUR 369.6 |
GRP78 / BiP Rabbit pAb |
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| A11366-200ul | Abclonal | 200 ul | EUR 550.8 |
GRP78 / BiP Rabbit pAb |
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| A11366-20ul | Abclonal | 20 ul | EUR 219.6 |
GRP78 / BiP Rabbit pAb |
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| A11366-50ul | Abclonal | 50 ul | EUR 267.6 |
GRP78 / BiP Rabbit pAb |
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| A11568-100ul | Abclonal | 100 ul | EUR 369.6 |
GRP78 / BiP Rabbit pAb |
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| A11568-200ul | Abclonal | 200 ul | EUR 550.8 |
GRP78 / BiP Rabbit pAb |
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| A11568-20ul | Abclonal | 20 ul | EUR 219.6 |
GRP78 / BiP Rabbit pAb |
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| A11568-50ul | Abclonal | 50 ul | EUR 267.6 |
GRP78 / BiP Rabbit pAb |
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| A0241-100ul | Abclonal | 100 ul | EUR 369.6 |
GRP78 / BiP Rabbit pAb |
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| A0241-200ul | Abclonal | 200 ul | EUR 550.8 |
GRP78 / BiP Rabbit pAb |
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| A0241-20ul | Abclonal | 20 ul | EUR 219.6 |
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